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Literature summary extracted from

  • Smith, B.A.; ONeil, E.J.; Lampkins, A.J.; Johnson, J.R.; Lee, J.J.; Cole, E.L.; Smith, B.D.
    Evaluation of fluorescent phosphatidylserine substrates for the aminophospholipid flippase in mammalian cells (2012), J. Fluoresc., 22, 93-101.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
7.6.2.1 analysis phosphatidylserine-coumarin O-[[(2R)-2-[(9-[5-[7-(diethylamino)-2-oxo-2H-1-benzopyran-3-yl]-1H-1,2,3-triazol-4-yl]nonanoyl)oxy]-3-(hexadecanoyloxy)propoxy](hydroxy)phosphoryl]-L-serine exhibits bright fluorescence and good photostability. It should be useful as a blue-emitting fluorescent translocation substrate for extended imaging studies of flippase action in living cells using laser confocal microscopy Cricetulus griseus

Organism

EC Number Organism UniProt Comment Textmining
7.6.2.1 Cricetulus griseus
-
-
-
7.6.2.1 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.6.2.1 A-549 cell
-
 Homo sapiens
-
7.6.2.1 CHO cell
-
 Cricetulus griseus
-
7.6.2.1 V-79 cell
-
 Cricetulus griseus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.6.2.1 ATP + H2O + 7-nitrobenz-2-oxa-1,3-diazol-4-yl-phosphatidylserine/in
-
 Cricetulus griseus ADP + phosphate + 7-nitrobenz-2-oxa-1,3-diazol-4-yl-phosphatidylserine/out
-
 ?
7.6.2.1 ATP + H2O + 7-nitrobenz-2-oxa-1,3-diazol-4-yl-phosphatidylserine/in
-
 Homo sapiens ADP + phosphate + 7-nitrobenz-2-oxa-1,3-diazol-4-yl-phosphatidylserine/out
-
 ?
7.6.2.1 ATP + H2O + carboxyfluoresceinyl-phosphatidylserine/in
-
 Cricetulus griseus ADP + phosphate + carboxyfluoresceinyl-phosphatidylserine/out
-
 ?
7.6.2.1 ATP + H2O + carboxyfluoresceinyl-phosphatidylserine/in
-
 Homo sapiens ADP + phosphate + carboxyfluoresceinyl-phosphatidylserine/out
-
 ?
7.6.2.1 ATP + H2O + coumaryl-phosphatidylserine/in
-
 Cricetulus griseus ADP + phosphate + coumaryl-phosphatidylserine/out
-
 ?
7.6.2.1 ATP + H2O + coumaryl-phosphatidylserine/in
-
 Homo sapiens ADP + phosphate + coumaryl-phosphatidylserine/out
-
 ?
7.6.2.1 additional information the enzyme does not translocalize 7-nitrobenz-2-oxa-1,3-diazol-4-yl-phosphatidylcholine or coumaryl-phosphatidylcholine Cricetulus griseus ?
-
 ?
7.6.2.1 additional information the enzyme does not translocalize 7-nitrobenz-2-oxa-1,3-diazol-4-yl-phosphatidylcholine or coumaryl-phosphatidylcholine Homo sapiens ?
-
 ?
7.6.2.1 additional information phosphatidylserine derivatives, with either a neutral 7-nitrobenz-2-oxa-1,3-diazol-4-yl or a coumarin fluorophore appended to the 2-acyl chain, are translocated by aminophospholipid flippase. A phosphatidylserine conjugate containing a charged and polar carboxyfluorescein is not translocated and remains in the cell plasma membrane Cricetulus griseus ?
-
 ?
7.6.2.1 O-[[(2R)-2-[(9-[5-[7-(diethylamino)-2-oxo-2H-1-benzopyran-3-yl]-1H-1,2,3-triazol-4-yl]nonanoyl)oxy]-3-(hexadecanoyloxy)propoxy](hydroxy)phosphoryl]-L-serine/outer leaflet + ? coumarin-derivative of phosphatidylserine Cricetulus griseus O-[[(2R)-2-[(9-[5-[7-(diethylamino)-2-oxo-2H-1-benzopyran-3-yl]-1H-1,2,3-triazol-4-yl]nonanoyl)oxy]-3-(hexadecanoyloxy)propoxy](hydroxy)phosphoryl]-L-serine/cytoplasmic leaflet + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.6.2.1 aminophospholipid flippase
-
 Cricetulus griseus
7.6.2.1 aminophospholipid flippase
-
 Homo sapiens