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Literature summary extracted from

  • Martinez-Gomez, A.I.; Andujar-Sanchez, M.; Clemente-Jimenez, J.M.; Neira, J.L.; Rodriguez-Vico, F.; Martinez-Rodriguez, S.; Las Heras-Vazquez, F.J.
    N-Carbamoyl-beta-alanine amidohydrolase from Agrobacterium tumefaciens C58: a promiscuous enzyme for the production of amino acids (2011), J. Chromatogr. B, 879, 3277-3282.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.6 His-tagged version expressed in Escherichia coli Agrobacterium tumefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.6 0.07
-
 N-carbamoyl-alpha-amino-beta-alanine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 0.11
-
 N-carbamoyl-L-methionine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 0.26
-
 N-carbamoyl-L-alanine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 0.84
-
 N-acetyl-L-methionine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 0.89
-
 N-carbamoyl-L-phenylalanine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 1.69
-
 N-carbamoyl-5-aminopentanoate pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 2.14
-
 N-Carbamoyl-beta-alanine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 2.96
-
 N-acetyl-L-alanine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 3.44
-
 N-carbamoyl-beta-homoalanine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 4.75
-
 N-Carbamoyl-glycine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 5.17
-
 N-carbamoyl-GABA pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 6.01
-
 N-formyl-L-methionine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 6.5
-
 N-carbamoyl-alpha-methyl-beta-alanine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 6.55
-
 N-carbamoyl-homoisoserine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 6.94
-
 N-carbamoyl-gamma-amino-beta-hydroxybutyric acid pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 9.09
-
 N-carbamoyl-D-ornithine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 9.47
-
 N-carbamoyl-L-ornithine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 10.58
-
 N-carbamoyl-taurine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 20.15
-
 N-carbamoyl-ciliatine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 84.38
-
 N-carbamoyl-alpha-phenyl-beta-alanine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 169.2
-
 N-Acetyl-beta-alanine pH 8.0, 30°C Agrobacterium tumefaciens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.6 Ni2+
-
 Agrobacterium tumefaciens

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.6 Agrobacterium tumefaciens
-
-
-
3.5.1.6 Agrobacterium tumefaciens C58 / ATCC 33970
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.6 immobilized metal ion affinity chromatography, gel filtration Agrobacterium tumefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.6 N-acetyl-beta-alanine + H2O
-
 Agrobacterium tumefaciens ?
-
 ?
3.5.1.6 N-acetyl-L-alanine + H2O
-
 Agrobacterium tumefaciens ?
-
 ?
3.5.1.6 N-acetyl-L-methionine + H2O
-
 Agrobacterium tumefaciens ?
-
 ?
3.5.1.6 N-carbamoyl-5-aminopentanoate + H2O
-
 Agrobacterium tumefaciens 5-aminopentanoate + CO2 + NH3
-
 ?
3.5.1.6 N-carbamoyl-alpha-amino-beta-alanine + H2O
-
 Agrobacterium tumefaciens 2,3-diaminopropanoate + CO2 + NH3
-
 ?
3.5.1.6 N-carbamoyl-alpha-methyl-beta-alanine + H2O
-
 Agrobacterium tumefaciens 3-amino-2-methylpropanoic acid + CO2 + NH3
-
 ?
3.5.1.6 N-carbamoyl-alpha-phenyl-beta-alanine + H2O
-
 Agrobacterium tumefaciens 3-amino-2-phenylpropanoic acid + CO2 + NH3
-
 ?
3.5.1.6 N-carbamoyl-beta-alanine + H2O
-
 Agrobacterium tumefaciens beta-alanine + CO2 + NH3
-
 ?
3.5.1.6 N-carbamoyl-beta-alanine + H2O
-
 Agrobacterium tumefaciens C58 / ATCC 33970 beta-alanine + CO2 + NH3
-
 ?
3.5.1.6 N-carbamoyl-beta-homoalanine + H2O
-
 Agrobacterium tumefaciens beta-homoalanine + CO2 + NH3
-
 ?
3.5.1.6 N-carbamoyl-ciliatine + H2O
-
 Agrobacterium tumefaciens ciliatine + CO2 + NH3
-
 ?
3.5.1.6 N-carbamoyl-D-ornithine + H2O
-
 Agrobacterium tumefaciens D-ornithine + CO2 + NH3
-
 ?
3.5.1.6 N-carbamoyl-GABA + H2O
-
 Agrobacterium tumefaciens 4-aminobutanoic acid + CO2 + NH3
-
 ?
3.5.1.6 N-carbamoyl-gamma-amino-beta-hydroxybutyric acid + H2O
-
 Agrobacterium tumefaciens ?
-
 ?
3.5.1.6 N-carbamoyl-glycine + H2O
-
 Agrobacterium tumefaciens glycine + CO2 + NH3
-
 ?
3.5.1.6 N-carbamoyl-glycine + H2O
-
 Agrobacterium tumefaciens C58 / ATCC 33970 glycine + CO2 + NH3
-
 ?
3.5.1.6 N-carbamoyl-homoisoserine + H2O
-
 Agrobacterium tumefaciens homoisoserine + CO2 + NH3
-
 ?
3.5.1.6 N-carbamoyl-L-alanine + H2O
-
 Agrobacterium tumefaciens L-alanine + CO2 + NH3
-
 ?
3.5.1.6 N-carbamoyl-L-alanine + H2O
-
 Agrobacterium tumefaciens C58 / ATCC 33970 L-alanine + CO2 + NH3
-
 ?
3.5.1.6 N-carbamoyl-L-methionine + H2O
-
 Agrobacterium tumefaciens L-methionine + CO2 + NH3
-
 ?
3.5.1.6 N-carbamoyl-L-methionine + H2O
-
 Agrobacterium tumefaciens C58 / ATCC 33970 L-methionine + CO2 + NH3
-
 ?
3.5.1.6 N-carbamoyl-L-ornithine + H2O
-
 Agrobacterium tumefaciens L-ornithine + CO2 + NH3
-
 ?
3.5.1.6 N-carbamoyl-L-phenylalanine + H2O
-
 Agrobacterium tumefaciens L-phenylalanine + CO2 + NH3
-
 ?
3.5.1.6 N-carbamoyl-L-phenylalanine + H2O
-
 Agrobacterium tumefaciens C58 / ATCC 33970 L-phenylalanine + CO2 + NH3
-
 ?
3.5.1.6 N-carbamoyl-taurine + H2O
-
 Agrobacterium tumefaciens taurine + CO2 + NH3
-
 ?
3.5.1.6 N-formyl-L-methionine + H2O
-
 Agrobacterium tumefaciens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.6 N-carbamoyl-beta-alanine amidohydrolase
-
 Agrobacterium tumefaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.6 additional information
-
 Tm = 326.23 K, unfolding temperature of wild type holoenzyme (determination by circular dichroism) Agrobacterium tumefaciens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.6 0.01
-
 N-carbamoyl-D-ornithine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 0.08
-
 N-carbamoyl-L-ornithine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 0.13
-
 N-carbamoyl-alpha-phenyl-beta-alanine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 0.15
-
 N-acetyl-L-methionine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 0.15
-
 N-carbamoyl-alpha-amino-beta-alanine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 0.21
-
 N-carbamoyl-beta-homoalanine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 0.23
-
 N-carbamoyl-ciliatine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 0.89
-
 N-acetyl-L-alanine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 1.95
-
 N-carbamoyl-gamma-amino-beta-hydroxybutyric acid pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 2.28
-
 N-carbamoyl-5-aminopentanoate pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 3.93
-
 N-Acetyl-beta-alanine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 4.27
-
 N-carbamoyl-homoisoserine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 5.81
-
 N-carbamoyl-L-phenylalanine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 5.84
-
 N-carbamoyl-taurine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 6.27
-
 N-carbamoyl-L-alanine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 8.64
-
 N-carbamoyl-L-methionine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 21.88
-
 N-Carbamoyl-glycine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 23.03
-
 N-formyl-L-methionine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 24.03
-
 N-carbamoyl-GABA pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 24.39
-
 N-carbamoyl-alpha-methyl-beta-alanine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 25.71
-
 N-Carbamoyl-beta-alanine pH 8.0, 30°C Agrobacterium tumefaciens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.6 0.00154
-
 N-carbamoyl-alpha-phenyl-beta-alanine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 0.0017
-
 N-carbamoyl-L-ornithine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 0.00882
-
 N-carbamoyl-D-ornithine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 0.01
-
 N-carbamoyl-ciliatine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 0.02
-
 N-Acetyl-beta-alanine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 0.06
-
 N-carbamoyl-beta-homoalanine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 0.18
-
 N-acetyl-L-methionine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 0.28
-
 N-carbamoyl-gamma-amino-beta-hydroxybutyric acid pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 0.3
-
 N-acetyl-L-alanine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 0.55
-
 N-carbamoyl-taurine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 0.65
-
 N-carbamoyl-homoisoserine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 1.35
-
 N-carbamoyl-5-aminopentanoate pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 2.14
-
 N-carbamoyl-alpha-amino-beta-alanine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 3.7
-
 N-carbamoyl-alpha-methyl-beta-alanine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 3.83
-
 N-formyl-L-methionine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 4.61
-
 N-Carbamoyl-glycine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 4.65
-
 N-carbamoyl-GABA pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 6.53
-
 N-carbamoyl-L-phenylalanine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 12.01
-
 N-Carbamoyl-beta-alanine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 24.12
-
 N-carbamoyl-L-alanine pH 8.0, 30°C Agrobacterium tumefaciens
3.5.1.6 78.55
-
 N-carbamoyl-L-methionine pH 8.0, 30°C Agrobacterium tumefaciens