EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.304 | recombinant expression of His-tagged PhaC1Pp in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Pseudomonas putida |
2.3.1.304 | recombinant expression of His-tagged PhaECAv in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Allochromatium vinosum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.304 | additional information | the enzyme is used for in vitro synthesis of polyhydroxyalkanoates on a hydrophobic support, i.e. highly oriented pyrolytic graphite. Using PhaC1Pp and 3-hydroxyoctanoyl-CoA at room temperature, a poly(3-hydroxyoctanoate) [P(3HO)] film is formed on the hydrophobic support with a thickness of a few nanometers, as revealed by atomic force microscopy | Pseudomonas putida |
2.3.1.304 | additional information | the enzyme is used for in vitro synthesis of polyhydroxyalkanoates on a hydrophobic support, i.e. highly oriented pyrolytic graphite. Using PhaECAv and 3-hydroxyoctanoyl-CoA at room temperature, a poly(3-hydroxyoctanoate) [P(3HO)] film is formed on the hydrophobic support with a thickness of a few nanometers, as revealed by atomic force microscopy | Allochromatium vinosum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.304 | 3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n | Pseudomonas putida | - |
[(R)-3-hydroxyacyl]n+1 + CoA | - |
? | |
2.3.1.304 | 3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n | Allochromatium vinosum | - |
[(R)-3-hydroxyacyl]n+1 + CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.304 | Allochromatium vinosum | - |
- |
- |
2.3.1.304 | Pseudomonas putida | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.304 | recombinant His-tagged PhaC1Pp from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Pseudomonas putida |
2.3.1.304 | recombinant His-tagged PhaECAv from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Allochromatium vinosum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.304 | 3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n | - |
Pseudomonas putida | [(R)-3-hydroxyacyl]n+1 + CoA | - |
? | |
2.3.1.304 | 3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n | - |
Allochromatium vinosum | [(R)-3-hydroxyacyl]n+1 + CoA | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.304 | class II PHA synthase | - |
Pseudomonas putida |
2.3.1.304 | class III PHA synthase | - |
Allochromatium vinosum |
2.3.1.304 | PhaC1Pp | - |
Pseudomonas putida |
2.3.1.304 | PhaECAv | - |
Allochromatium vinosum |