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Literature summary extracted from

  • Sato, S.; Minato, M.; Kikkawa, Y.; Abe, H.; Tsuge, T.
    In vitro synthesis of polyhydroxyalkanoate catalyzed by class II and III PHA synthases: A useful technique for surface coatings of a hydrophobic support with PHA (2010), J. Chem. Technol. Biotechnol., 85, 779-782.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.304 recombinant expression of His-tagged PhaC1Pp in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Pseudomonas putida
2.3.1.304 recombinant expression of His-tagged PhaECAv in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Allochromatium vinosum

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.304 additional information the enzyme is used for in vitro synthesis of polyhydroxyalkanoates on a hydrophobic support, i.e. highly oriented pyrolytic graphite. Using PhaC1Pp and 3-hydroxyoctanoyl-CoA at room temperature, a poly(3-hydroxyoctanoate) [P(3HO)] film is formed on the hydrophobic support with a thickness of a few nanometers, as revealed by atomic force microscopy Pseudomonas putida
2.3.1.304 additional information the enzyme is used for in vitro synthesis of polyhydroxyalkanoates on a hydrophobic support, i.e. highly oriented pyrolytic graphite. Using PhaECAv and 3-hydroxyoctanoyl-CoA at room temperature, a poly(3-hydroxyoctanoate) [P(3HO)] film is formed on the hydrophobic support with a thickness of a few nanometers, as revealed by atomic force microscopy Allochromatium vinosum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.304 3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n Pseudomonas putida
-
[(R)-3-hydroxyacyl]n+1 + CoA
-
?
2.3.1.304 3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n Allochromatium vinosum
-
[(R)-3-hydroxyacyl]n+1 + CoA
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.304 Allochromatium vinosum
-
-
-
2.3.1.304 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.304 recombinant His-tagged PhaC1Pp from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Pseudomonas putida
2.3.1.304 recombinant His-tagged PhaECAv from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Allochromatium vinosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.304 3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n
-
Pseudomonas putida [(R)-3-hydroxyacyl]n+1 + CoA
-
?
2.3.1.304 3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n
-
Allochromatium vinosum [(R)-3-hydroxyacyl]n+1 + CoA
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.304 class II PHA synthase
-
Pseudomonas putida
2.3.1.304 class III PHA synthase
-
Allochromatium vinosum
2.3.1.304 PhaC1Pp
-
Pseudomonas putida
2.3.1.304 PhaECAv
-
Allochromatium vinosum