EC Number | Application | Comment | Organism |
---|---|---|---|
2.1.3.3 | biotechnology | HepG2 is an immortalized human hepatoma cell line that has been used for research into bioartificial liver systems. However, a low level of ammonia detoxification is its biggest drawback. Stable overexpression of arginase I and ornithine transcarbamylase in HepG2 cells improves its ammonia detoxification | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.3.3 | stable overexpression of arginase I and ornithine transcarbamylase in HepG2 cells using a eukaryotic dual gene expression vector pBudCE4.1, the co-expression improves ammonia detoxification of the cells, improvement of HepG2 cell function, overview | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.1.3.3 | mitochondrion | - |
Homo sapiens | 5739 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.3.3 | carbamoyl phosphate + L-ornithine | Homo sapiens | - |
L-citrulline + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.3.3 | Homo sapiens | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.3.3 | Hep-G2 cell | - |
Homo sapiens | - |
2.1.3.3 | hepatocyte | primary | Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.3.3 | carbamoyl phosphate + L-ornithine | - |
Homo sapiens | L-citrulline + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.3.3 | ornithine transcarbamylase | - |
Homo sapiens |
2.1.3.3 | OTC | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.3.3 | 37 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.3.3 | 7.4 | - |
assay at | Homo sapiens |