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Literature summary extracted from

  • Dai, X.; Mashiguchi, K.; Chen, Q.; Kasahara, H.; Kamiya, Y.; Ojha, S.; Dubois, J.; Ballou, D.; Zhao, Y.
    The biochemical mechanism of auxin biosynthesis by an Arabidopsis YUCCA flavin-containing monooxygenase (2013), J. Biol. Chem., 288, 1448-1457.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.168 expession in Escherichia coli Arabidopsis thaliana
1.14.13.168 expressed in Escherichia coli BL21 Star (DE3) cells Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.168 0.026
-
 NADPH in the presence of phenylpyruvate, in 50 mM sodium phosphate, pH 8.0, 500 mM NaCl, and 30% (v/v) glycerol, at 25°C Arabidopsis thaliana
1.14.13.168 0.043
-
 phenylpyruvate in the presence of NADPH, in 50 mM sodium phosphate, pH 8.0, 500 mM NaCl, and 30% (v/v) glycerol, at 25°C Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.168 48000
-
 x * 48000, isoform YUC6, SDS-PAGE Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.168 Arabidopsis thaliana
-
-
-
1.14.13.168 Arabidopsis thaliana Q8VZ59
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.168 (indol-3-yl)pyruvate + NADPH + H+ + O2
-
 Arabidopsis thaliana (indol-3-yl)acetate + NADP+ + H2O + CO2
-
 ?
1.14.13.168 (indol-3-yl)pyruvate + NADPH + H+ + O2
-
 Arabidopsis thaliana (indol-3-yl)acetate + NADP+ + H2O + CO2 first step of the YUC6-catalyzed reaction is the reduction of the FAD cofactor to FADH- by NADPH. Subsequently, FADH- reacts with oxygen to form a flavin-C4a-(hydro)peroxy intermediate, which has a maximum absorbance at 381 nm in its UV-visible spectrum. The final chemical step is the reaction of the C4a-intermediate with (indol-3-yl)pyruvate to produce (indol-3-yl)acetate. The YUC6 intermediate has a half-life of about 20 s ?
1.14.13.168 phenylpyruvate + NADPH + H+ + O2 isoform YUC6 shows basal NADPH oxidase activity in the absence of the cosubstrate phenylpyruvate Arabidopsis thaliana phenylacetate + NADP+ + H2O + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.168 ? x * 48000, isoform YUC6, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.14.13.168 At5g25620 locus name Arabidopsis thaliana
1.14.13.168 YUC6
-
 Arabidopsis thaliana
1.14.13.168 YUC6 isoform Arabidopsis thaliana
1.14.13.168 YUCCA
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.168 0.31
-
 NADPH in the presence of phenylpyruvate, in 50 mM sodium phosphate, pH 8.0, 500 mM NaCl, and 30% (v/v) glycerol, at 25°C Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.168 FAD
-
 Arabidopsis thaliana
1.14.13.168 FAD isoform YUC6 contains oxidized flavin as a cofactor Arabidopsis thaliana
1.14.13.168 additional information isoform YUC6 does not accept FMN as cofactor Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
1.14.13.168 physiological function the enzyme catalyzes a rate-limiting step in auxin biosynthesis and is essential for many developmental processes Arabidopsis thaliana