EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.3.18 | expressed in Escherichia coli C41 OverExpress and B834(DE3) cells | Abies grandis |
4.2.3.132 | DELTA84 pseudo-mature form of abietadiene synthase is expressed in Escherichia coli C41 and B834(DE3) cells | Abies grandis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.2.3.18 | hanging drop vapor diffusion method, using 24% (v/v) PEG 8000, 0.1 M sodium citrate, pH 5.1, 0.1 M dibasic ammonium phosphate | Abies grandis |
4.2.3.132 | hanging drop vapor diffusion method, using 24% (w/v) PEG 8000, 0.1 M sodium citrate, pH 5.1, 0.1 M dibasic ammonium phosphate | Abies grandis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.3.18 | N451A | this mutation reduces class II (protonation-initiated cyclization) activity about 100fold, with essentially no effect on class I (ionization-initiated cyclization) activity | Abies grandis |
4.2.3.132 | D621A | the mutation selectively abrogates abietadiene synthase class I activity | Abies grandis |
4.2.3.132 | N451A | the mutant shows reduced class I abietadiene synthase activity compared to the wild type enzyme | Abies grandis |
4.2.3.132 | R356A | the mutant shows reduced class I abietadiene synthase activity compared to the wild type enzyme | Abies grandis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.3.132 | 0.0007 | - |
(+)-copalyl diphosphate | mutant enzyme R356A, pH and temperature not specified in the publication | Abies grandis | |
4.2.3.132 | 0.0014 | - |
(+)-copalyl diphosphate | mutant enzyme N451A, pH and temperature not specified in the publication | Abies grandis | |
4.2.3.132 | 0.002 | - |
(+)-copalyl diphosphate | wild type enzyme, pH and temperature not specified in the publication | Abies grandis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.18 | (+)-copalyl diphosphate | Abies grandis | - |
abieta-7,13-diene + diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.3.18 | Abies grandis | Q38710 | - |
- |
4.2.3.132 | Abies grandis | Q38710 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.3.18 | Mono Q column chromatography and type II ceramic hydroxyapatite column chromatography | Abies grandis |
4.2.3.132 | type II ceramic hydroxyapatite column chromatography and Mono Q column chromatography | Abies grandis |
EC Number | Storage Stability | Organism |
---|---|---|
4.2.3.18 | -80°C, purified enzyme in 10 mM Bis-Tris, pH 6.8, 10% (v/v) glycerol,150 mM KCl, 10 mM MgCl2, and 5 mM dithiohtreitol, several months, without significant loss of activity | Abies grandis |
4.2.3.132 | -80°C, 10 mM Bis-Tris, pH 6.8, 10% (v/v) glycerol, 150 mM KCl, 10 mM MgCl2, and 5 mM dithiothreitol, several months, without significant loss of activity | Abies grandis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.18 | (+)-copalyl diphosphate | - |
Abies grandis | abieta-7,13-diene + diphosphate | - |
? | |
4.2.3.132 | (+)-copalyl diphosphate | - |
Abies grandis | neoabietadiene + diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.3.132 | homotetramer | x-ray crystallography | Abies grandis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.3.18 | abietadiene synthase | - |
Abies grandis |
4.2.3.132 | abietadiene synthase | cf. EC 4.2.3.18 | Abies grandis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.3.132 | 1.1 | - |
(+)-copalyl diphosphate | mutant enzyme R356A, pH and temperature not specified in the publication | Abies grandis | |
4.2.3.132 | 1.9 | - |
(+)-copalyl diphosphate | mutant enzyme N451A, pH and temperature not specified in the publication | Abies grandis | |
4.2.3.132 | 2.6 | - |
(+)-copalyl diphosphate | wild type enzyme, pH and temperature not specified in the publication | Abies grandis |