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Literature summary extracted from

  • Pollak, A.J.; Reich, N.O.
    Proximal recognition sites facilitate intrasite hopping by DNA adenine methyltransferase: Mechanistic exploration of epigenetic gene regulation (2012), J. Biol. Chem., 287, 22873-22881.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.72 expressed in Escherichia coli XL2Blue cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.72 N126A the mutant shows large reduction in methylation activity Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.72 Escherichia coli
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Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.72 phosphocellulose column chromatography and Blue Sepharose column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme methylates adenines at the N6 position of palindromic 5’-GATC-3’ sites. The enzyme ethylates both strands of the same site prior to fully dissociating from the DNA, a process referred to as intrasite processivity. Intrasite processivity is disrupted when the DNA flanking a single GATC site is longer than 400 bp on either side. The introduction of a second GATC site within this flanking DNA reinstates intrasite methylation of both sites Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methyladenine
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Synonyms

EC Number Synonyms Comment Organism
2.1.1.72 Dam
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 Escherichia coli
2.1.1.72 DNA adenine methyltransferase
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 Escherichia coli