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Literature summary extracted from

  • Senavirathne, G.; Jaszczur, M.; Auerbach, P.A.; Upton, T.G.; Chelico, L.; Goodman, M.F.; Rueda, D.
    Single-stranded DNA scanning and deamination by APOBEC3G cytidine deaminase at single molecule resolution (2012), J. Biol. Chem., 287, 15826-15835.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.4.B9 additional information large increase in the mobility of APOBEC3G cytidine deaminase on ssDNA at higher salt, Mg2+ or NaCl Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.B9 Mg2+ large increase in the mobility of APOBEC3G cytidine deaminase on ssDNA at higher salt, Mg2+ or NaCl Homo sapiens
3.5.4.B9 NaCl large increase in the mobility of APOBEC3G cytidine deaminase on ssDNA at higher salt, Mg2+ or NaCl Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.B9 cytosine in single-stranded viral DNA + H2O Homo sapiens phage M13mp2 circular DNA containing a series of in-frame 5'-AAACCCAAA hot motifs embedded in lacZalpha reporter sequence located within a single-stranded gapped region of M13 double-stranded DNA. The third C in the 5'-AAACCCAAA motif is deaminated predominantly uracil in single-stranded viral DNA + NH3
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Organism

EC Number Organism UniProt Comment Textmining
3.5.4.B9 Homo sapiens
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-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.B9 cytosine in single-stranded viral DNA + H2O phage M13mp2 circular DNA containing a series of in-frame 5'-AAACCCAAA hot motifs embedded in lacZalpha reporter sequence located within a single-stranded gapped region of M13 double-stranded DNA. The third C in the 5'-AAACCCAAA motif is deaminated predominantly Homo sapiens uracil in single-stranded viral DNA + NH3
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 ?
3.5.4.B9 additional information APOBEC3G cytidine deaminase contracts ssDNA in a deamination motif-dependent manner, presence of bidirectional quasi-localized scanning of APOBEC3G cytidine deaminase occurring proximal to a 5' hot motif, a motif-dependent DNA contraction greatest for 5' hot before 3' hot before 5' cold motifs, and diminished mobility at low salt, overview Homo sapiens ?
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 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.B9 More APOBEC3G cytidine deaminase catalytically inactive N-terminal CD1 domain has a predicted large net positive charge, in contrast to the catalytically active CD2 domain, and is likely to govern the mobility of APOBEC3G cytidine deaminase on ssDNA, which should depend on metal ion concentration Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.5.4.B9 Apo3G
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 Homo sapiens
3.5.4.B9 APOBEC3G
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 Homo sapiens
3.5.4.B9 APOBEC3G cytidine deaminase
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 Homo sapiens

General Information

EC Number General Information Comment Organism
3.5.4.B9 physiological function the single-stranded DNA-dependent cytosine deaminase inactivates HIV-1 in T cells by C to T hypermutation Homo sapiens
3.5.4.14 additional information mechanistic studies on ssDNA scanning enzymes, investigation of ssDNA scanning and motif-targeting mechanisms for APOBEC3G cytidine deaminase using single molecule Förster resonance energy transfer, overview Homo sapiens
3.5.4.14 physiological function APOBEC3G is a single-stranded DNA cytosine deaminase that eliminates HIV-1 infectivity by converting C to U in numerous small target motifs on the minus viral cDNA. APOBEC3G cytidine deaminase deaminates linear ssDNA in vitro with pronounced spatial asymmetry favoring the 3' to 5' direction. A similar polarity observed in vivo is believed responsible for initiating localized C to T mutational gradients that inactivate the virus Homo sapiens