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Literature summary extracted from

  • Evjenth, R.H.; Brenner, A.K.; Thompson, P.R.; Arnesen, T.; Fr?ystein, N.A.; Lillehaug, J.R.
    Human protein N-terminal acetyltransferase hNaa50p (hNAT5/hSAN) follows ordered sequential catalytic mechanism: combined kinetic and NMR study (2012), J. Biol. Chem., 287, 10081-10088.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.258 H112A inactive Homo sapiens
2.3.1.258 H112A NMR spectroscopy using the catalytically inactive hNaa50p mutant Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.258 CoA competitive product inhibition; product inhibition, competitive versus acetyl-CoA, noncompetitive against peptide substrate well in line with a ternary complex mechanism. The non-competitive inhibition pattern observed when CoA is tested against peptide as variable substrate and at fixed concentrations of acetyl-CoA also rules out a ping-pong mechanism Homo sapiens
2.3.1.258 desulfo-CoA competitive inhibition; competitive versus acetyl-CoA Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.258 additional information
-
additional information steady-state kinetics, overview Homo sapiens
2.3.1.258 0.005
-
acetyl-CoA pH 7.4, temperature not specified in the publication Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.258 acetyl-CoA + peptide Homo sapiens
-
CoA + Nalpha-acetylpeptide
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.258 Homo sapiens
-
-
-
2.3.1.258 Homo sapiens Q9GZZ1
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.258 acetyl-CoA + an N-terminal-L-methionyl-L-leucyl-[protein] = an N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-[protein] + CoA hNaa50p utilizes an ordered bi bi reaction of the Theorell-Chance type, substarte binding study and dynamics using NMR spectroscopy. Acetyl-CoA induces a conformational change that is required for the peptide to bind to the active site. Addition of peptide in the absence of acetyl-CoA does not alter the structure of the protein Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.258 acetyl-CoA + MLGPEGGRWGRPVGRRRRPVRVYP optimal in vitro substrate Homo sapiens ?
-
?
2.3.1.258 acetyl-CoA + peptide
-
Homo sapiens CoA + Nalpha-acetylpeptide
-
?
2.3.1.258 additional information Naa50p also possesses Nepsilon-autoacetylation activity Homo sapiens ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.258 Naa50p
-
Homo sapiens
2.3.1.258 NAT5/SAN
-
Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.258 7.4 8 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.258 acetyl-CoA acetyl-CoA induces a conformational change that is required for the peptide to bind to the active site Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.258 0.00227
-
CoA with acetyl-CoA, pH 7.4, temperature not specified in the publication Homo sapiens
2.3.1.258 0.0027
-
CoA pH and temperature not specified in the publication Homo sapiens
2.3.1.258 0.00277
-
CoA with peptide, pH 7.4, temperature not specified in the publication Homo sapiens
2.3.1.258 0.067
-
desulfo-CoA pH and temperature not specified in the publication Homo sapiens
2.3.1.258 0.067
-
desulfo-CoA with acetyl-CoA, pH 7.4, temperature not specified in the publication Homo sapiens