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Literature summary extracted from

  • Yang, M.T.; Chang, C.H.; Wang, J.M.; Wu, T.K.; Wang, Y.K.; Chang, C.Y.; Li, T.T.
    Crystal structure and inhibition studies of transglutaminase from Streptomyces mobaraense (2011), J. Biol. Chem., 286, 7301-7307.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.13 expressed in Escherichia coli BL21(DE3) cells Streptomyces mobaraensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.2.13 hanging drop vapor diffusion method, using 30% (w/v) PEG 8000, 50 mM NaCl, 1 mM EDTA, 1 mM 2-mercaptoethanol, 0.01% (w/v) NaN3, 100 mM cacodylic acid (pH 5.0), and 2% (v/v) glycerol Streptomyces mobaraensis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.13 D301A the mutation drastically reduces the catalytic activity of the enzyme Streptomyces mobaraensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.13 GLGQGETYR
-
 Streptomyces mobaraensis
2.3.2.13 SQAETQR
-
 Streptomyces mobaraensis
2.3.2.13 SQAETYR noncompetitive inhibition Streptomyces mobaraensis
2.3.2.13 SYAETYR noncompetitive inhibition Streptomyces mobaraensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.13 52.66
-
 Nalpha-benzyloxycarbonyl-L-glutaminylglycine in 250 mM Tris acetate buffer (pH 8.0), at 37°C Streptomyces mobaraensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.13 Nalpha-benzyloxycarbonyl-L-glutaminylglycine + hydroxylamine Streptomyces mobaraensis
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.13 Streptomyces mobaraensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.13 Ni-NTA column chromatography Streptomyces mobaraensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.13 Nalpha-benzyloxycarbonyl-L-glutaminylglycine + hydroxylamine
-
 Streptomyces mobaraensis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.13 MTGase
-
 Streptomyces mobaraensis
2.3.2.13 TGase
-
 Streptomyces mobaraensis
2.3.2.13 transglutaminase
-
 Streptomyces mobaraensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.2.13 35.48
-
 Nalpha-benzyloxycarbonyl-L-glutaminylglycine in 250 mM Tris acetate buffer (pH 8.0), at 37°C Streptomyces mobaraensis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.2.13 0.65
-
 in 250 mM Tris acetate buffer (pH 8.0), at 37°C Streptomyces mobaraensis SQAETYR
2.3.2.13 0.75
-
 in 250 mM Tris acetate buffer (pH 8.0), at 37°C Streptomyces mobaraensis SYAETYR

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.2.13 0.67
-
 Nalpha-benzyloxycarbonyl-L-glutaminylglycine in 250 mM Tris acetate buffer (pH 8.0), at 37°C Streptomyces mobaraensis