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Literature summary extracted from

  • Lee, S.G.; Haakenson, W.; McCarter, J.P.; Williams, D.J.; Hresko, M.C.; Jez, J.M.
    Thermodynamic evaluation of ligand binding in the plant-like phosphoethanolamine methyltransferases of the parasitic nematode Haemonchus contortus (2011), J. Biol. Chem., 286, 38060-38068.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.103 expressed in Escherichia coli Rosetta II(DE3) pLysS cells Haemonchus contortus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.103 amodiaquine i.e. 4-[(7-chloroquinolin-4-yl)amino]-2-[(diethylamino)methyl]phenol Haemonchus contortus
2.1.1.103 diphenhydramine i.e. 2-(diphenylmethoxy)-N,N-dimethylethanamine Haemonchus contortus
2.1.1.103 miltefosine i.e. hexadecyl 2-(trimethylammonio)ethyl phosphate Haemonchus contortus
2.1.1.103 additional information isozyme PMT1 is not inhibited by diphenhydramine Haemonchus contortus
2.1.1.103 sinefungin
-
 Haemonchus contortus
2.1.1.103 tacrine i.e. 1,2,3,4-tetrahydroacridin-9-amine Haemonchus contortus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.103 0.106
-
 Phosphoethanolamine isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus
2.1.1.103 0.217
-
 phosphodimethylethanolamine isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus
2.1.1.103 0.256
-
 S-adenosyl-L-methionine isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus
2.1.1.103 0.277
-
 S-adenosyl-L-methionine isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus
2.1.1.103 0.424
-
 phosphomonomethylethanolamine isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.103 48000
-
 1 * 48000, isozyme PMT2, SDS-PAGE Haemonchus contortus
2.1.1.103 49400
-
 1 * 49400, isozyme PMT2, calculated from amino acid sequence Haemonchus contortus
2.1.1.103 51900
-
 isozyme PMT2, gel filtration Haemonchus contortus
2.1.1.103 52300
-
 isozyme PMT1, gel filtration Haemonchus contortus
2.1.1.103 53100
-
 1 * 53100, isozyme PMT1, calculated from amino acid sequence Haemonchus contortus
2.1.1.103 55000
-
 1 * 55000, isozyme PMT1, SDS-PAGE Haemonchus contortus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.103 Haemonchus contortus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.103 Ni2+-nitrilotriacetic acid-agarose resin column chromatography and Superdex-200 gel filtration Haemonchus contortus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.103 3 S-adenosyl-L-methionine + phosphoethanolamine overall reaction Haemonchus contortus 3 S-adenosyl-L-homocysteine + phosphocholine
-
 ?
2.1.1.103 additional information isozyme PMT2 does not accept phosphoethanolamine as substrate Haemonchus contortus ?
-
 ?
2.1.1.103 S-adenosyl-L-methionine + phosphodimethylethanolamine reaction catalyzed by isozyme PMT2 Haemonchus contortus S-adenosyl-L-homocysteine + phosphocholine
-
 ?
2.1.1.103 S-adenosyl-L-methionine + phosphoethanolamine reaction catalyzed by isozyme PMT1 Haemonchus contortus S-adenosyl-L-homocysteine + phosphomonomethylethanolamine
-
 ?
2.1.1.103 S-adenosyl-L-methionine + phosphomonomethylethanolamine reaction catalyzed by isozyme PMT2 Haemonchus contortus S-adenosyl-L-homocysteine + phosphodimethylethanolamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.103 monomer 1 * 48000, isozyme PMT2, SDS-PAGE Haemonchus contortus
2.1.1.103 monomer 1 * 49400, isozyme PMT2, calculated from amino acid sequence Haemonchus contortus
2.1.1.103 monomer 1 * 53100, isozyme PMT1, calculated from amino acid sequence Haemonchus contortus
2.1.1.103 monomer 1 * 55000, isozyme PMT1, SDS-PAGE Haemonchus contortus

Synonyms

EC Number Synonyms Comment Organism
2.1.1.103 phosphoethanolamine methyltransferase
-
 Haemonchus contortus
2.1.1.103 PMT1 isozyme PMT1 contains a methyltransferase domain in the N-terminal half of the protein Haemonchus contortus
2.1.1.103 PMT2 isozyme PMT2 encodes a C-terminal methyltransferase domain Haemonchus contortus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.103 2.28
-
 S-adenosyl-L-methionine isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus
2.1.1.103 4.13
-
 phosphodimethylethanolamine isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus
2.1.1.103 4.9
-
 phosphomonomethylethanolamine isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus
2.1.1.103 5.82
-
 S-adenosyl-L-methionine isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus
2.1.1.103 8.63
-
 Phosphoethanolamine isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.103 Haemonchus contortus isozyme PMT1, calculated from amino acid sequence
-
 5.3
2.1.1.103 Haemonchus contortus isozyme PMT2, calculated from amino acid sequence
-
 5.5

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.1.1.103 0.0125
-
 isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus miltefosine
2.1.1.103 0.013
-
 isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus diphenhydramine
2.1.1.103 0.0338
-
 isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus sinefungin
2.1.1.103 0.0915
-
 isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus sinefungin
2.1.1.103 0.1252
-
 isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus miltefosine
2.1.1.103 3.1
-
 isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus amodiaquine
2.1.1.103 3.4
-
 isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus amodiaquine
2.1.1.103 3.5
-
 isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus tacrine
2.1.1.103 23.4
-
 isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus tacrine

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.103 8.24
-
 S-adenosyl-L-methionine isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus
2.1.1.103 11.56
-
 phosphomonomethylethanolamine isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus
2.1.1.103 19.05
-
 phosphodimethylethanolamine isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus
2.1.1.103 22.72
-
 S-adenosyl-L-methionine isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus
2.1.1.103 81.45
-
 Phosphoethanolamine isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C Haemonchus contortus