Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Spears, J.L.; Rubio, M.A.; Gaston, K.W.; Wywial, E.; Strikoudis, A.; Bujnicki, J.M.; Papavasiliou, F.N.; Alfonzo, J.D.
    A single zinc ion is sufficient for an active Trypanosoma brucei tRNA editing deaminase (2011), J. Biol. Chem., 286, 20366-20374.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.5.4.33 C136A kcat/Km is 13% of the wild-type value, mutation in subunit ADAT2 Trypanosoma brucei
3.5.4.33 C136A/ADAT3 C291A inactive mutant enzyme, mutation in subunit ADAT2 Trypanosoma brucei
3.5.4.33 C139A inactive mutant enzyme, mutation in subunit ADAT2 Trypanosoma brucei
3.5.4.33 C291A inactive mutant enzyme, mutation in subunit ADAT3 Trypanosoma brucei
3.5.4.33 C294A inactive mutant enzyme, mutation in subunit ADAT3 Trypanosoma brucei
3.5.4.33 E92A inactive mutant enzyme, mutation in subunit ADAT2 Trypanosoma brucei
3.5.4.33 H252A inactive mutant enzyme, mutation in subunit ADAT3 Trypanosoma brucei
3.5.4.33 H90A inactive mutant enzyme, mutation in subunit ADAT2 Trypanosoma brucei
3.5.4.33 V254E inactive mutant enzyme, mutation in subunit ADAT3 Trypanosoma brucei
3.5.4.33 V254L kcat/Km is 7% of the wild-type value, mutation in subunit ADAT3 Trypanosoma brucei
3.5.4.33 V254T kcat/Km is 20% of the wild-type value, mutation in subunit ADAT3 Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.33 0.00075
-
 adenine34 in tRNAVal 27°C, pH 8.0, wild-type enzyme Trypanosoma brucei
3.5.4.33 0.0015
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme C136A, mutation in subunit ADAT2 Trypanosoma brucei
3.5.4.33 0.0021
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme V254T, mutation in subunit ADAT2 Trypanosoma brucei
3.5.4.33 0.0028
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme V254L, mutation in subunit ADAT3 Trypanosoma brucei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.33 Zn2+ wild type enzyme coordinates two Zn2+ per heterodimer. Mutation of one of the key Zn2+-coordinating cysteines in TbADAT2 yields a functional enzyme with a single bound zinc Trypanosoma brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.33 adenine34 in tRNAVal + H2O Trypanosoma brucei
-
 hypoxanthine34 in tRNAVal + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.33 Trypanosoma brucei
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.33 adenine34 in tRNAVal + H2O
-
 Trypanosoma brucei hypoxanthine34 in tRNAVal + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.4.33 ADAT2/3
-
 Trypanosoma brucei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.33 27
-
 assay at Trypanosoma brucei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.4.33 0.00083
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme C136A, mutation in subunit ADAT2 Trypanosoma brucei
3.5.4.33 0.00083
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme V254L, mutation in subunit ADAT3 Trypanosoma brucei
3.5.4.33 0.0018
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme V254T, mutation in subunit ADAT2 Trypanosoma brucei
3.5.4.33 0.0032
-
 adenine34 in tRNAVal 27°C, pH 8.0, wild-type enzyme Trypanosoma brucei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.33 8
-
 assay at Trypanosoma brucei

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.4.33 0.3
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme V254L, mutation in subunit ADAT3 Trypanosoma brucei
3.5.4.33 0.55
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme C136A, mutation in subunit ADAT2 Trypanosoma brucei
3.5.4.33 0.86
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme V254T, mutation in subunit ADAT2 Trypanosoma brucei
3.5.4.33 4.3
-
 adenine34 in tRNAVal 27°C, pH 8.0, wild-type enzyme Trypanosoma brucei