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Literature summary extracted from

  • Soontharapirakkul, K.; Promden, W.; Yamada, N.; Kageyama, H.; Incharoensakdi, A.; Iwamoto-Kihara, A.; Takabe, T.
    Halotolerant cyanobacterium Aphanothece halophytica contains an Na+-dependent F1F0-ATP synthase with a potential role in salt-stress tolerance (2011), J. Biol. Chem., 286, 10169-10176.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.2.2 expressed in Escherichia coli mutant DK8 deficient in ATP synthase and in Synechococcus sp. strain PCC 7942 Aphanothece halophytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.2.2 1,3-dicyclohexyl carbodiimide
-
 Aphanothece halophytica
7.1.2.2 monensin 70% inhibition at 0.1 mM; 70% inhibition at 0.1 mM; 70% inhibition at 0.1 mM Aphanothece halophytica
7.1.2.2 additional information not inhibited by carbonyl cyanide m-chlorophenyl hydrazine; not inhibited by carbonyl cyanide m-chlorophenyl hydrazine; not inhibited by carbonyl cyanide m-chlorophenyl hydrazine Aphanothece halophytica
7.1.2.2 N,NĀ’-dicyclohexylcarbodiimide
-
 Aphanothece halophytica
7.1.2.2 Tributyltin chloride 80% inhibition at 0.1 mM; 80% inhibition at 0.1 mM; 80% inhibition at 0.1 mM Aphanothece halophytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.2.2 2.8
-
 ATP apparent value, recombinant enzyme, in 20 mM Tris-HCl, pH 7.6, 5mM MgCl2, 10 mM NaCl, temperature not specified in the publication Aphanothece halophytica
7.1.2.2 3
-
 Na+/in apparent value, recombinant enzyme, in 20 mM Tris-HCl, pH 7.6, 5mM MgCl2, 10 mM NaCl, temperature not specified in the publication Aphanothece halophytica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.2.2 cytoplasmic membrane
-
 Aphanothece halophytica
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.2.2 Na+ ApNa+-ATPase has a putative Na+-binding site in subunit epsilon. The Na+ ion protects the inhibition of Na+-ATPase by N,NĀ’-dicyclohexylcarbodiimide Aphanothece halophytica
7.1.2.2 Na+ the Na+ ion protects the inhibition of Na+-ATPase by N,NĀ’-dicyclohexylcarbodiimide Aphanothece halophytica

Organism

EC Number Organism UniProt Comment Textmining
7.1.2.2 Aphanothece halophytica F2Z9M7 epsilon subunit
-
7.1.2.2 Aphanothece halophytica F2Z9M8 I subunit
-
7.1.2.2 Aphanothece halophytica F2Z9N4 gamma subunit
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.2.2 ATP + H2O + Na+/in
-
 Aphanothece halophytica ADP + phosphate + Na+/out
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.1.2.2 ApNa+-ATPase
-
 Aphanothece halophytica
7.1.2.2 Na+-dependent F1F0-ATP synthase
-
 Aphanothece halophytica

General Information

EC Number General Information Comment Organism
7.1.2.2 physiological function Na+-dependent F1F0-ATP synthase in the cytoplasmic membrane plays a potential role in salt-stress tolerance Aphanothece halophytica