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Literature summary extracted from

  • Batt, S.M.; Jabeen, T.; Mishra, A.K.; Veerapen, N.; Krumbach, K.; Eggeling, L.; Besra, G.S.; Fuetterer, K.
    Acceptor substrate discrimination in phosphatidyl-myo-inositol mannoside synthesis: structural and mutational analysis of mannosyltransferase Corynebacterium glutamicum PimB (2010), J. Biol. Chem., 285, 37741-37752.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.346 expressed in Escherichia coli BL21(DE3) Corynebacterium glutamicum
2.4.1.346 expression in Escherichia coli Corynebacterium glutamicum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.346 PEG 3350, pH 5.5 Corynebacterium glutamicum
2.4.1.346 structure of mannosyltransferase PimB in complex with nucleotide to a resolution of 2.0 A. Both the nucleotide conformation as well as the structural framework of the active site display flexibility Corynebacterium glutamicum

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.346 D13A 1.9% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
2.4.1.346 D13A 1.9% of wild-type activity Corynebacterium glutamicum
2.4.1.346 D13N 1.5% of wild-type activity Corynebacterium glutamicum
2.4.1.346 D13N 1.5% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
2.4.1.346 D13Y 0.3% of wild-type activity Corynebacterium glutamicum
2.4.1.346 D13Y 0.3% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
2.4.1.346 E290D 2.9% of wild-type activity Corynebacterium glutamicum
2.4.1.346 E290D 2.9% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
2.4.1.346 E290N 1.1% of wild-type activity Corynebacterium glutamicum
2.4.1.346 E290N 1.1% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
2.4.1.346 E290Q 4.6% of wild-type activity Corynebacterium glutamicum
2.4.1.346 E290Q 4.6% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
2.4.1.346 G123P 86.6% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
2.4.1.346 G123P 86.6% of wild-type activity Corynebacterium glutamicum
2.4.1.346 G20W 3.2% of wild-type activity Corynebacterium glutamicum
2.4.1.346 G20W 3.2% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
2.4.1.346 G291S 5.3% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
2.4.1.346 G291S 5.3% of wild-type activity Corynebacterium glutamicum
2.4.1.346 H118S 0.7% of wild-type activity Corynebacterium glutamicum
2.4.1.346 H118S 0.7% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
2.4.1.346 H120S 81.2% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
2.4.1.346 H120S 81.2% of wild-type activity Corynebacterium glutamicum
2.4.1.346 I21A 2.0% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
2.4.1.346 I21A 2.0% of wild-type activity Corynebacterium glutamicum
2.4.1.346 I21S 2.4% of wild-type activity Corynebacterium glutamicum
2.4.1.346 I21S 2.4% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
2.4.1.346 K211Q 3.5% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
2.4.1.346 K211Q 3.5% of wild-type activity Corynebacterium glutamicum
2.4.1.346 N12A 31.2% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
2.4.1.346 N12A 31.2% of wild-type activity Corynebacterium glutamicum
2.4.1.346 Q22A 14.3% of wild-type activity Corynebacterium glutamicum
2.4.1.346 Q22A 14.3% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
2.4.1.346 R206S 5.6% of wild-type activity Corynebacterium glutamicum
2.4.1.346 R206S 5.6% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
2.4.1.346 R210S 8.7% of wild-type activity Corynebacterium glutamicum
2.4.1.346 R210S 8.7% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
2.4.1.346 S205G 119.6% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
2.4.1.346 S205G 119.6% of wild-type activity Corynebacterium glutamicum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.346 Corynebacterium glutamicum
-
-
-
2.4.1.346 Corynebacterium glutamicum Q8NNK8
-
-
2.4.1.346 Corynebacterium glutamicum DSM 20300 Q8NNK8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.346 immobilized metal ion affinity chromatography (Ni2+) Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.346 GDP-alpha-D-mannopyranose + phosphatidyl-myo-inositol attaches mannosyl selectively to the 6-OH of the inositol moiety Corynebacterium glutamicum GDP + ?
-
 ?
2.4.1.346 GDP-alpha-D-mannose + 2-O-(6-O-acyl-alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol
-
 Corynebacterium glutamicum GDP + 2-O-(6-O-acyl-alpha-D-mannosyl)-6-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
-
 ?
2.4.1.346 GDP-alpha-D-mannose + 2-O-(6-O-acyl-alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol
-
 Corynebacterium glutamicum DSM 20300 GDP + 2-O-(6-O-acyl-alpha-D-mannosyl)-6-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
-
 ?
2.4.1.346 GDP-alpha-D-mannose + 2-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
-
 Corynebacterium glutamicum GDP + 2,6-di-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
-
 ?
2.4.1.346 GDP-alpha-D-mannose + 2-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
-
 Corynebacterium glutamicum DSM 20300 GDP + 2,6-di-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
-
 ?
2.4.1.346 additional information PimB attaches the mannosyl moiety selectively to the 6-OH of the inositol moiety of phosphatidylinositol Corynebacterium glutamicum ?
-
 ?
2.4.1.346 additional information PimB attaches the mannosyl moiety selectively to the 6-OH of the inositol moiety of phosphatidylinositol Corynebacterium glutamicum DSM 20300 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.346 mannosyltransferase PimB'
-
 Corynebacterium glutamicum
2.4.1.346 PimB
-
 Corynebacterium glutamicum