Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sun, T.; Hayakawa, K.; Bateman, K.S.; Fraser, M.E.
    Identification of the citrate-binding site of human ATP-citrate lyase using X-ray crystallography (2010), J. Biol. Chem., 285, 27418-27428.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.8 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.3.8 in complex with citrate or tartrate, hanging drop vapor diffusion method, using either 12.5% (w/v) PEG 3350, 100 mM sodium tartrate, 100 mM Tris-HCl (pH 7.0) for the native protein or 10% PEG 3350, 75 mM potassium citrate, 100 mM Tris-HCl (pH 7.0) for the selenomethionyl protein Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.8 Tartrate
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.3.8 cytoplasm
-
 Homo sapiens 5737
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.8 Homo sapiens P53396
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.8 Ni-NTA agarose column chromatography, Q-Sepharose column chromatography and DEAE-Sephacel gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.8 ATP + citrate + CoA
-
 Homo sapiens ADP + phosphate + acetyl-CoA + oxaloacetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.3.8 ACLY
-
 Homo sapiens
2.3.3.8 ATP-citrate lyase
-
 Homo sapiens