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Literature summary extracted from

  • Veiga-da-Cunha, M.; Tyteca, D.; Stroobant, V.; Courtoy, P.J.; Opperdoes, F.R.; Van Schaftingen, E.
    Molecular identification of NAT8 as the enzyme that acetylates cysteine S-conjugates to mercapturic acids (2010), J. Biol. Chem., 285, 18888-18898.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.80 octyl glucoside 1 mM, slight stimulation, inhibitory at higher concentration Homo sapiens

Application

EC Number Application Comment Organism
2.3.1.80 medicine the frequently found single nucleotide polymorphisms, E104K or F143S, do not change enzymic activity or the expression of protein by more than 2fold Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.80 expression in HEK-293T cell Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.80 E104K frequently found single nucleotide polymorphism, does not change enzymic activity or the expression of protein by more than 2fold Homo sapiens
2.3.1.80 F143S frequently found single nucleotide polymorphism, does not change enzymic activity or the expression of protein by more than 2fold Homo sapiens
2.3.1.80 additional information a truncated protein starting from residue M25 shows no cysteinyl-S-conjugate N-acetyltransferase activity Homo sapiens
2.3.1.80 R149K suppression of activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.80 5,5'-dithiobis-2-nitrobenzoic acid strong inhibition Homo sapiens
2.3.1.80 octyl glucoside 10 mM, 80% residual activity. Slight activation at 1 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.80 0.023
-
 acetyl-CoA pH 7.5, 37°C Homo sapiens
2.3.1.80 0.064
-
 S-Benzyl-L-cysteine pH 7.5, 37°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.80 29000
-
 x * 29000, SDS-PAGE of recombinant protein including His-tag Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.80 Homo sapiens Q9UHE5
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.80 kidney
-
 Homo sapiens
-
2.3.1.80 liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.80 acetyl-CoA + leukotriene E4 about 1% of the activity with S-benzyl-L-cysteine Homo sapiens CoA + N-acetyl-leukotriene E4
-
 ?
2.3.1.80 acetyl-CoA + S-benzyl-L-cysteine
-
 Homo sapiens CoA + N-acetyl-S-benzyl-L-cysteine
-
 ?
2.3.1.80 additional information besides S-benzyl-L-cysteine and leukotriene E4, enzyme is inactive on physiological amines or amino acids L-aspartate, L-lysine, L-cystine, L-methionine, L-ethionine, L-cystathionine, or L-phenylalanine, L-tyrosine, and L-tryptophan Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.80 ? x * 29000, SDS-PAGE of recombinant protein including His-tag Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.3.1.80 NAT8
-
 Homo sapiens