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Literature summary extracted from

  • Rigbers, O.; Li, S.M.
    Ergot alkaloid biosynthesis in Aspergillus fumigatus. Overproduction and biochemical characterization of a 4-dimethylallyltryptophan N-methyltransferase (2008), J. Biol. Chem., 283, 26859-26868.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.261 gene fgaMT, DNA and amino acid sequence determination and analysis, overexpression of the soluble His6-tagged enzyme in Escherichia coli strain XL-1 blue MRF- Aspergillus fumigatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.261 EDTA 13% inhibition at 5 mM Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.261 0.12
-
4-dimethylallyl-L-tryptophan pH 7.5, 37°C, recombinant enzyme Aspergillus fumigatus
2.1.1.261 2.4
-
S-adenosyl-L-methionine pH 7.5, 37°C, recombinant enzyme Aspergillus fumigatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.261 additional information the enzyme does not require metal ions Mg2+,Mn2+, Ca2+, K+, and Na+ for its enzymatic reaction Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.261 38100
-
2 * 40000, recombinant His6-tagged enzyme, SDS-PAGE, x * 39200, about, His6-tagged enzyme, sequence calculation, x * 38100, about, non-tagged enzyme, sequence calculation Aspergillus fumigatus
2.1.1.261 39200
-
2 * 40000, recombinant His6-tagged enzyme, SDS-PAGE, x * 39200, about, His6-tagged enzyme, sequence calculation, x * 38100, about, non-tagged enzyme, sequence calculation Aspergillus fumigatus
2.1.1.261 40000
-
2 * 40000, recombinant His6-tagged enzyme, SDS-PAGE, x * 39200, about, His6-tagged enzyme, sequence calculation, x * 38100, about, non-tagged enzyme, sequence calculation Aspergillus fumigatus
2.1.1.261 75000
-
recombinant His6-tagged enzyme, gel filtration Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.261 S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan Aspergillus fumigatus
-
S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
-
?
2.1.1.261 S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan Aspergillus fumigatus B5233
-
S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.261 Aspergillus fumigatus B6D5I7 gene fgaMT, ATCC 13073
-
2.1.1.261 Aspergillus fumigatus B5233 B6D5I7 gene fgaMT, ATCC 13073
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.261 recombinant soluble His6-tagged enzyme in Escherichia coli strain XL-1 blue MRF- by nickel affinity chromatography and gel filtration to apparent homogeneity Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.261 additional information the enzyme shows a relatively high specificity toward the prenyl moiety at position C-4 of the indole ring, 4-dimethylallyltryptophan derivatives with modification at the indole ring are also accepted by FgaMT as substrates Aspergillus fumigatus ?
-
?
2.1.1.261 additional information the enzyme shows a relatively high specificity toward the prenyl moiety at position C-4 of the indole ring, 4-dimethylallyltryptophan derivatives with modification at the indole ring are also accepted by FgaMT as substrates Aspergillus fumigatus B5233 ?
-
?
2.1.1.261 S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan
-
Aspergillus fumigatus S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
-
?
2.1.1.261 S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan the enzyme shows a relatively high specificity toward the prenyl moiety at position C-4 of the indole ring Aspergillus fumigatus S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine product identificaation by NMR and mass spectrometry analysis ?
2.1.1.261 S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan
-
Aspergillus fumigatus B5233 S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
-
?
2.1.1.261 S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan the enzyme shows a relatively high specificity toward the prenyl moiety at position C-4 of the indole ring Aspergillus fumigatus B5233 S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine product identificaation by NMR and mass spectrometry analysis ?

Subunits

EC Number Subunits Comment Organism
2.1.1.261 homodimer 2 * 40000, recombinant His6-tagged enzyme, SDS-PAGE, x * 39200, about, His6-tagged enzyme, sequence calculation, x * 38100, about, non-tagged enzyme, sequence calculation Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
2.1.1.261 fgaMT
-
Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.261 37
-
assay at Aspergillus fumigatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.261 2
-
S-adenosyl-L-methionine pH 7.5, 37°C, recombinant enzyme Aspergillus fumigatus
2.1.1.261 2
-
4-dimethylallyl-L-tryptophan pH 7.5, 37°C, recombinant enzyme Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.261 7.5
-
assay at Aspergillus fumigatus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.261 S-adenosyl-L-methionine
-
Aspergillus fumigatus

General Information

EC Number General Information Comment Organism
2.1.1.261 metabolism FgaMT represents the second pathway-specific enzyme in the biosynthesis of ergot alkaloids Aspergillus fumigatus