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Literature summary extracted from

  • Ohnuma, M.; Terui, Y.; Tamakoshi, M.; Mitome, H.; Niitsu, M.; Samejima, K.; Kawashima, E.; Oshima, T.
    N1-aminopropylagmatine, a new polyamine produced as a key intermediate in polyamine biosynthesis of an extreme thermophile, Thermus thermophilus (2005), J. Biol. Chem., 280, 30073-30082.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.104 expression in Escherichia coli Thermus thermophilus
3.5.3.24 overexpression in Escherichia coli Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.104 0.77
-
 agmatine pH 9.0, 37°C Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.104 32500
-
 x * 32500, SDS-PAGE Thermus thermophilus
3.5.3.24 32500
-
 x * 32500, SDS-PAGE Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.104 S-adenosyl 3-(methylthio)propylamine + agmatine Thermus thermophilus the enzyme is involved in polyamine biosynthesis 5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
 ?
3.5.3.24 N1-aminopropylagmatine + H2O Thermus thermophilus the enzyme is involved in biosynthesis of spermidine. The conversion of N1-aminopropylagmatine to spermidine is essential for production of long and branched polyamines in Thermus thermophilus spermidine + urea
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.104 Thermus thermophilus Q5SK28
-
-
3.5.3.24 Thermus thermophilus Q5SJ85
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.104
-
 Thermus thermophilus
3.5.3.24
-
 Thermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.104 2.4
-
 pH 8.5, 60°C Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.104 S-adenosyl 3-(methylthio)propylamine + agmatine
-
 Thermus thermophilus 5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
 ?
2.5.1.104 S-adenosyl 3-(methylthio)propylamine + agmatine the enzyme is involved in polyamine biosynthesis Thermus thermophilus 5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
 ?
3.5.3.24 N1-aminopropylagmatine + H2O the enzyme is involved in biosynthesis of spermidine. The conversion of N1-aminopropylagmatine to spermidine is essential for production of long and branched polyamines in Thermus thermophilus Thermus thermophilus spermidine + urea
-
 ?
3.5.3.24 N1-aminopropylagmatine + H2O the enzyme cannot use agmatine as substrate Thermus thermophilus spermidine + urea
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.104 ? x * 32500, SDS-PAGE Thermus thermophilus
3.5.3.24 ? x * 32500, SDS-PAGE Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
2.5.1.104 agmatine aminopropyltransferase
-
 Thermus thermophilus
2.5.1.104 SpeE
-
 Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.104 37
-
 assay at Thermus thermophilus
3.5.3.24 65
-
 assay at Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.104 0.37
-
 agmatine pH 9.0, 37°C Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.104 9
-
 assay at Thermus thermophilus
3.5.3.24 7.4
-
 assay at Thermus thermophilus

General Information

EC Number General Information Comment Organism
3.5.3.24 malfunction a disruption mutant accumulates N1-aminopropylagmatine Thermus thermophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.104 480
-
 agmatine pH 9.0, 37°C Thermus thermophilus