BRENDA - Enzyme Database

N1-aminopropylagmatine, a new polyamine produced as a key intermediate in polyamine biosynthesis of an extreme thermophile, Thermus thermophilus

Ohnuma, M.; Terui, Y.; Tamakoshi, M.; Mitome, H.; Niitsu, M.; Samejima, K.; Kawashima, E.; Oshima, T.; J. Biol. Chem. 280, 30073-30082 (2005)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.5.1.104
expression in Escherichia coli
Thermus thermophilus
3.5.3.24
overexpression in Escherichia coli
Thermus thermophilus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5.1.104
0.77
-
agmatine
pH 9.0, 37°C
Thermus thermophilus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.5.1.104
32500
-
x * 32500, SDS-PAGE
Thermus thermophilus
3.5.3.24
32500
-
x * 32500, SDS-PAGE
Thermus thermophilus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + agmatine
Thermus thermophilus
the enzyme is involved in polyamine biosynthesis
5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
-
?
3.5.3.24
N1-aminopropylagmatine + H2O
Thermus thermophilus
the enzyme is involved in biosynthesis of spermidine. The conversion of N1-aminopropylagmatine to spermidine is essential for production of long and branched polyamines in Thermus thermophilus
spermidine + urea
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.5.1.104
Thermus thermophilus
Q5SK28
-
-
3.5.3.24
Thermus thermophilus
Q5SJ85
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.5.1.104
-
Thermus thermophilus
3.5.3.24
-
Thermus thermophilus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.5.1.104
2.4
-
pH 8.5, 60°C
Thermus thermophilus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + agmatine
-
719832
Thermus thermophilus
5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
-
-
?
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + agmatine
the enzyme is involved in polyamine biosynthesis
719832
Thermus thermophilus
5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
-
-
?
3.5.3.24
N1-aminopropylagmatine + H2O
the enzyme is involved in biosynthesis of spermidine. The conversion of N1-aminopropylagmatine to spermidine is essential for production of long and branched polyamines in Thermus thermophilus
719832
Thermus thermophilus
spermidine + urea
-
-
-
?
3.5.3.24
N1-aminopropylagmatine + H2O
the enzyme cannot use agmatine as substrate
719832
Thermus thermophilus
spermidine + urea
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.5.1.104
?
x * 32500, SDS-PAGE
Thermus thermophilus
3.5.3.24
?
x * 32500, SDS-PAGE
Thermus thermophilus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.5.1.104
37
-
assay at
Thermus thermophilus
3.5.3.24
65
-
assay at
Thermus thermophilus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.5.1.104
0.37
-
agmatine
pH 9.0, 37°C
Thermus thermophilus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.104
9
-
assay at
Thermus thermophilus
3.5.3.24
7.4
-
assay at
Thermus thermophilus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.5.1.104
expression in Escherichia coli
Thermus thermophilus
3.5.3.24
overexpression in Escherichia coli
Thermus thermophilus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5.1.104
0.77
-
agmatine
pH 9.0, 37°C
Thermus thermophilus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.5.1.104
32500
-
x * 32500, SDS-PAGE
Thermus thermophilus
3.5.3.24
32500
-
x * 32500, SDS-PAGE
Thermus thermophilus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + agmatine
Thermus thermophilus
the enzyme is involved in polyamine biosynthesis
5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
-
?
3.5.3.24
N1-aminopropylagmatine + H2O
Thermus thermophilus
the enzyme is involved in biosynthesis of spermidine. The conversion of N1-aminopropylagmatine to spermidine is essential for production of long and branched polyamines in Thermus thermophilus
spermidine + urea
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.5.1.104
-
Thermus thermophilus
3.5.3.24
-
Thermus thermophilus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.5.1.104
2.4
-
pH 8.5, 60°C
Thermus thermophilus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + agmatine
-
719832
Thermus thermophilus
5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
-
-
?
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + agmatine
the enzyme is involved in polyamine biosynthesis
719832
Thermus thermophilus
5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
-
-
?
3.5.3.24
N1-aminopropylagmatine + H2O
the enzyme is involved in biosynthesis of spermidine. The conversion of N1-aminopropylagmatine to spermidine is essential for production of long and branched polyamines in Thermus thermophilus
719832
Thermus thermophilus
spermidine + urea
-
-
-
?
3.5.3.24
N1-aminopropylagmatine + H2O
the enzyme cannot use agmatine as substrate
719832
Thermus thermophilus
spermidine + urea
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.5.1.104
?
x * 32500, SDS-PAGE
Thermus thermophilus
3.5.3.24
?
x * 32500, SDS-PAGE
Thermus thermophilus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.5.1.104
37
-
assay at
Thermus thermophilus
3.5.3.24
65
-
assay at
Thermus thermophilus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.5.1.104
0.37
-
agmatine
pH 9.0, 37°C
Thermus thermophilus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.104
9
-
assay at
Thermus thermophilus
3.5.3.24
7.4
-
assay at
Thermus thermophilus
General Information
EC Number
General Information
Commentary
Organism
3.5.3.24
malfunction
a disruption mutant accumulates N1-aminopropylagmatine
Thermus thermophilus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.5.3.24
malfunction
a disruption mutant accumulates N1-aminopropylagmatine
Thermus thermophilus
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.5.1.104
480
-
agmatine
pH 9.0, 37°C
Thermus thermophilus
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.5.1.104
480
-
agmatine
pH 9.0, 37°C
Thermus thermophilus