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Literature summary extracted from
- The biochemical characterization of two carotenoid cleavage enzymes from Arabidopsis indicates that a carotenoid-derived compound inhibits lateral branching (2004), J. Biol. Chem., 279, 46940-46945.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.68 | methanol | addition of methanol to in vitro assays has a stimulatory effect on activity at concentrations up to 25% | Arabidopsis thaliana |  |
| 1.13.11.71 | methanol | addition of methanol to in vitro assays has a stimulatory effect on activity at concentrations up to 25% | Arabidopsis thaliana | |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.13.11.68 | additional information | a truncated enzyme protein of 587 amino acids lacking the N-terminal targeting sequence is also able to catalyze the 910 cleavage of beta-carotene. Coexpression of the enzyme, CCD7, and all-trans-10'-apo-beta-carotenal 13,14-cleaving dioxygenase CCD8, EC 1.13.11.70, in Escherichia coli results in production of 13-apo-beta-carotenone. The sequential cleavages of beta-carotene by CCD7 and CCD8 are likely the initial steps in the synthesis of a carotenoid-derived signaling molecule that is necessary for the regulation lateral branching | Arabidopsis thaliana |
| 1.13.11.69 | additional information | coexpression of the enzyme, CCD8, and carotenoid-9',10'-cleaving dioxygenase CCD7, EC 1.13.11.71, in Escherichia coli results in production of 13-apo-beta-carotenone. The sequential cleavages of beta-carotene by CCD7 and CCD8 are likely the initial steps in the synthesis of a carotenoid-derived signaling molecule that is necessary for the regulation lateral branching | Arabidopsis thaliana |
| 1.13.11.70 | additional information | coexpression of the enzyme, CCD8, and carotenoid-9',10'-cleaving dioxygenase CCD7, EC 1.13.11.71, in Escherichia coli results in production of 13-apo-beta-carotenone. The sequential cleavages of beta-carotene by CCD7 and CCD8 are likely the initial steps in the synthesis of a carotenoid-derived signaling molecule that is necessary for the regulation lateral branching | Arabidopsis thaliana |
| 1.13.11.71 | additional information | a truncated enzyme protein of 587 amino acids lacking the N-terminal targeting sequence is also able to catalyze the 910 cleavage of beta-carotene. Coexpression of the enzyme, CCD7, and all-trans-10'-apo-beta-carotenal 13,14-cleaving dioxygenase CCD8, EC 1.13.11.70, in Escherichia coli results in production of 13-apo-beta-carotenone. The sequential cleavages of beta-carotene by CCD7 and CCD8 are likely the initial steps in the synthesis of a carotenoid-derived signaling molecule that is necessary for the regulation lateral branching | Arabidopsis thaliana |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.68 | 0.0152 | - |
beta-carotene | pH 7.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.13.11.71 | 0.0152 | - |
beta-carotene | pH 7.0, temperature not specified in the publication | Arabidopsis thaliana | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.13.11.68 | chloroplast | protein contains a probable chloroplast targeting sequence of 31 amino acids | Arabidopsis thaliana | 9507 | - |
| 1.13.11.71 | chloroplast | protein contains a probable chloroplast targeting sequence of 31 amino acids | Arabidopsis thaliana | 9507 | - |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 1.13.11.68 | Methanol | addition of methanol to in vitro assays has a stimulatory effect on activity at concentrations up to 25% | Arabidopsis thaliana |
| 1.13.11.71 | Methanol | addition of methanol to in vitro assays has a stimulatory effect on activity at concentrations up to 25% | Arabidopsis thaliana |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.68 | Arabidopsis thaliana | Q7XJM2 | - |
- |
| 1.13.11.69 | Arabidopsis thaliana | - |
- |
- |
| 1.13.11.70 | Arabidopsis thaliana | - |
- |
- |
| 1.13.11.71 | Arabidopsis thaliana | Q7XJM2 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.68 | beta-carotene + O2 | - |
Arabidopsis thaliana | 10'-apo-beta-carotenal + beta-ionone | - |
? | |
| 1.13.11.68 | additional information | no substrate: lycopene, lutein, zeaxanthin, violaxanthin, or neoxanthin | Arabidopsis thaliana | ? | - |
? | |
| 1.13.11.71 | beta-carotene + O2 | - |
Arabidopsis thaliana | 10'-apo-beta-carotenal + beta-ionone | - |
? | |
| 1.13.11.71 | additional information | no substrate: lycopene, lutein, zeaxanthin, violaxanthin, or neoxanthin | Arabidopsis thaliana | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.13.11.68 | CCD7 | - |
Arabidopsis thaliana |
| 1.13.11.69 | CCD8 | - |
Arabidopsis thaliana |
| 1.13.11.69 | MAX4 | - |
Arabidopsis thaliana |
| 1.13.11.70 | CCD8 | - |
Arabidopsis thaliana |
| 1.13.11.70 | MAX4 | - |
Arabidopsis thaliana |
| 1.13.11.71 | CCD7 | - |
Arabidopsis thaliana |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.13.11.68 | physiological function | coexpression of the enzyme, CCD7, and all-trans-10'-apo-beta-carotenal 13,14-cleaving dioxygenase CCD8, EC 1.13.11.70, in Escherichia coli results in production of 13-apo-beta-carotenone. The sequential cleavages of beta-carotene by CCD7 and CCD8 are likely the initial steps in the synthesis of a carotenoid-derived signaling molecule that is necessary for the regulation lateral branching | Arabidopsis thaliana |
| 1.13.11.69 | physiological function | coexpression of the enzyme, CCD8, and carotenoid-9',10'-cleaving dioxygenase CCD7, EC 1.13.11.71, in Escherichia coli results in production of 13-apo-beta-carotenone. The sequential cleavages of beta-carotene by CCD7 and CCD8 are likely the initial steps in the synthesis of a carotenoid-derived signaling molecule that is necessary for the regulation lateral branching | Arabidopsis thaliana |
| 1.13.11.70 | physiological function | coexpression of the enzyme, CCD8, and carotenoid-9',10'-cleaving dioxygenase CCD7, EC 1.13.11.71, in Escherichia coli results in production of 13-apo-beta-carotenone. The sequential cleavages of beta-carotene by CCD7 and CCD8 are likely the initial steps in the synthesis of a carotenoid-derived signaling molecule that is necessary for the regulation lateral branching | Arabidopsis thaliana |
| 1.13.11.71 | physiological function | coexpression of the enzyme, CCD7, and all-trans-10'-apo-beta-carotenal 13,14-cleaving dioxygenase CCD8, EC 1.13.11.70, in Escherichia coli results in production of 13-apo-beta-carotenone. The sequential cleavages of beta-carotene by CCD7 and CCD8 are likely the initial steps in the synthesis of a carotenoid-derived signaling molecule that is necessary for the regulation lateral branching | Arabidopsis thaliana |
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