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Literature summary extracted from

  • Mills, J.A.; Motichka, K.; Jucker, M.; Wu, H.P.; Uhlik, B.C.; Stern, R.J.; Scherman, M.S.; Vissa, V.D.; Pan, F.; Kundu, M.; Ma, Y.F.; McNeil, M.
    Inactivation of the mycobacterial rhamnosyltransferase, which is needed for the formation of the arabinogalactan-peptidoglycan linker, leads to irreversible loss of viability (2004), J. Biol. Chem., 279, 43540-43546.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.289 gene wbbL, expression in and complementation of Mycobacterium smegmatis strain mc2155 temperature-sensitive mutant 2-20/32 Escherichia coli
2.4.1.289 orf Rv3265c or gene wbbL, expression in and complementation of Mycobacterium smegmatis strain mc2155 temperature-sensitive mutant 2-20/32, the enzyme also is capable of restoring an Escherichia coli K12 strain containing an insertionally inactivated wbbL to O-antigen positive, functional expression from plasmid in Escherichia coli strain K12 EMG2 Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.289 additional information the Escherichia coli mutant strain K12 EMG2 contains an insertionally inactivated wbbL and is devoid of any O-antigen and shows no WbbL activity, complementation by expression of WbbL from Mycobacterium tuberculosis, a plasmid encoding the putative rhamnosyltransferase gene from Mycobacterium tuberculosis also results in O-antigen synthesis Escherichia coli
2.4.1.289 additional information the temperature-sensitive mutant contains a single-base change that has a proline changed to a serine residue. Exposure of in mc2155 mutant 2-20/32 to higher non-permissive temperatures results in bacteria that cannot be recovered at the lower permissive temperatures. Complementation of the mutants strain by expression of gene wbbL from Escherichia coli or Mycobacterium tuberculosis Mycolicibacterium smegmatis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.289 Escherichia coli P36667 gene wbbL
-
2.4.1.289 Escherichia coli WG1 P36667 gene wbbL
-
2.4.1.289 Mycobacterium tuberculosis P9WMY3 orf Rv3265c, gene wbbL
-
2.4.1.289 Mycobacterium tuberculosis H37Rv P9WMY3 orf Rv3265c, gene wbbL
-
2.4.1.289 Mycolicibacterium smegmatis Q9RN49 a temperature-sensitive mutant strain 2-20/32, gene wbbL
-
2.4.1.289 Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 Q9RN49 a temperature-sensitive mutant strain 2-20/32, gene wbbL
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.289 dTDP-beta-L-rhamnose + GlcNAc-diphospho-trans,octacis-decaprenol
-
 Mycobacterium tuberculosis ?
-
 ?
2.4.1.289 dTDP-beta-L-rhamnose + GlcNAc-diphospho-trans,octacis-decaprenol
-
 Mycobacterium tuberculosis H37Rv ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.289 dTDP-Rha:alpha-D-GlcNAc-pyrophosphate polyprenol, alpha-3-L-rhamnosyltransferase
-
 Mycobacterium tuberculosis
2.4.1.289 dTDP-Rha:alpha-D-GlcNAc-pyrophosphate polyprenol, alpha-3-L-rhamnosyltransferase
-
 Mycolicibacterium smegmatis
2.4.1.289 dTDP-Rha:alpha-D-GlcNAc-pyrophosphate polyprenol, alpha-3-L-rhamnosyltransferase
-
 Escherichia coli
2.4.1.289 WbbL
-
 Mycobacterium tuberculosis
2.4.1.289 WbbL
-
 Mycolicibacterium smegmatis
2.4.1.289 WbbL
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.289 37
-
 assay at Mycobacterium tuberculosis
2.4.1.289 37
-
 assay at Mycolicibacterium smegmatis
2.4.1.289 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.289 8
-
 assay at Mycobacterium tuberculosis
2.4.1.289 8
-
 assay at Mycolicibacterium smegmatis
2.4.1.289 8
-
 assay at Escherichia coli

General Information

EC Number General Information Comment Organism
2.4.1.289 malfunction the temperature-sensitive mc2155 strain 2-20/32 mutant shows loss of this transferase activity in 2-20/32 at elevated temperatures, complementation by expression of the enzyme encoded by genes wbbL from Escherichia coli and froms Mycobacterium tuberculosis Mycolicibacterium smegmatis
2.4.1.289 physiological function the enzyme is required for the rhamnosyl-L-containing linker unit responsible for the attachment of the cell wall polymer mycolyl-arabinogalactan to the peptidoglycan in mycobacteria Mycobacterium tuberculosis