EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.2.54 | overexpression in Escherichia coli | Methanocaldococcus jannaschii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.54 | 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate | uncompetitive inhibition under varied 5-phospho-alpha-D-ribose 1-diphosphate and saturated 4-aminobenzoate or under varied 4-aminobenzoate and saturated 5-phospho-alpha-D-ribose 1-diphosphate | Methanocaldococcus jannaschii | |
2.4.2.54 | CO2 | uncompetitive inhibition under varied 5-phospho-alpha-D-ribose 1-diphosphate and saturated 4-aminobenzoate. Mixed type of inhibition under varied 4-aminobenzoate and saturated 5-phospho-alpha-D-ribose 1-diphosphate | Methanocaldococcus jannaschii | |
2.4.2.54 | diphosphate | competitive inhibition under saturated 5-phospho-alpha-D-ribose 1-diphosphate and varied 4-aminobenzoate. Mixed type of inhibition under varied 4-aminobenzoate and saturated 5-phospho-alpha-D-ribose 1-diphosphate | Methanocaldococcus jannaschii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.54 | 0.15 | - |
4-Aminobenzoate | pH 4.8, 70°C | Methanocaldococcus jannaschii | |
2.4.2.54 | 1.5 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 4.8, 70°C | Methanocaldococcus jannaschii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.2.54 | 36200 | - |
2 * 36200, SDS-PAGE | Methanocaldococcus jannaschii |
2.4.2.54 | 70000 | - |
gel filtration | Methanocaldococcus jannaschii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.54 | 5-phospho-alpha-D-ribose 1-diphosphate + 4-aminobenzoate | Methanocaldococcus jannaschii | the enzyme is involved in biosynthesis of tetrahydromethanopterin, a folate analog involved in the C1 metabolism of methanogenic archaea, sulfate-reducing archaea, and methylotrophic bacteria | 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.54 | Methanocaldococcus jannaschii | Q58822 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.2.54 | - |
Methanocaldococcus jannaschii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.2.54 | 0.248 | - |
pH 4.8, 70°C | Methanocaldococcus jannaschii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.54 | 5-phospho-alpha-D-ribose 1-diphosphate + 4-aminobenzoate | the enzyme is involved in biosynthesis of tetrahydromethanopterin, a folate analog involved in the C1 metabolism of methanogenic archaea, sulfate-reducing archaea, and methylotrophic bacteria | Methanocaldococcus jannaschii | 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate | - |
? | |
2.4.2.54 | 5-phospho-alpha-D-ribose 1-diphosphate + 4-aminobenzoate | ordered Bi-Ter mechanism involving binding of 5-phospho-alpha-D-ribose 1-diphosphate, then 4-aminobenzoate, followed by release of the products CO2, then 4-(beta-D-ribofuranosyl)aminobenzene-5'-phosphate, and finally diphosphate | Methanocaldococcus jannaschii | 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.2.54 | homodimer | 2 * 36200, SDS-PAGE | Methanocaldococcus jannaschii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.2.54 | beta-RFA-P synthase | - |
Methanocaldococcus jannaschii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.54 | 70 | - |
assay at | Methanocaldococcus jannaschii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.54 | 0.23 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 4.8, 70°C | Methanocaldococcus jannaschii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.2.54 | 4.9 | - |
- |
Methanocaldococcus jannaschii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.54 | additional information | the enzyme lacks any chromogenic cofactor, and the presence of pyridoxal phosphate and the mechanistically related pyruvoyl cofactors is excluded | Methanocaldococcus jannaschii |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.54 | 0.21 | - |
4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate | pH 4.8, 70°C, uncompetitive inhibition under varied 5-phospho-alpha-D-ribose 1-diphosphate and saturated 4-aminobenzoate | Methanocaldococcus jannaschii | |
2.4.2.54 | 0.3 | - |
4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate | pH 4.8, 70°C, uncompetitive inhibition under varied 4-aminobenzoate and saturated 5-phospho-alpha-D-ribose 1-diphosphate | Methanocaldococcus jannaschii | |
2.4.2.54 | 0.32 | - |
diphosphate | pH 4.8, 70°C, competitive inhibition under saturated 5-phospho-alpha-D-ribose 1-diphosphate and varied 4-aminobenzoate | Methanocaldococcus jannaschii | |
2.4.2.54 | 0.99 | - |
CO2 | pH 4.8, 70°C, uncompetitive inhibition under varied 5-phospho-alpha-D-ribose 1-diphosphate and saturated 4-aminobenzoate | Methanocaldococcus jannaschii |