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Literature summary extracted from

  • Dumitru, R.V.; Ragsdale, S.W.
    Mechanism of 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate synthase, a key enzyme in the methanopterin biosynthetic pathway (2004), J. Biol. Chem., 279, 39389-39395.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.54 overexpression in Escherichia coli Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.54 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate uncompetitive inhibition under varied 5-phospho-alpha-D-ribose 1-diphosphate and saturated 4-aminobenzoate or under varied 4-aminobenzoate and saturated 5-phospho-alpha-D-ribose 1-diphosphate Methanocaldococcus jannaschii
2.4.2.54 CO2 uncompetitive inhibition under varied 5-phospho-alpha-D-ribose 1-diphosphate and saturated 4-aminobenzoate. Mixed type of inhibition under varied 4-aminobenzoate and saturated 5-phospho-alpha-D-ribose 1-diphosphate Methanocaldococcus jannaschii
2.4.2.54 diphosphate competitive inhibition under saturated 5-phospho-alpha-D-ribose 1-diphosphate and varied 4-aminobenzoate. Mixed type of inhibition under varied 4-aminobenzoate and saturated 5-phospho-alpha-D-ribose 1-diphosphate Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.54 0.15
-
4-Aminobenzoate pH 4.8, 70°C Methanocaldococcus jannaschii
2.4.2.54 1.5
-
5-phospho-alpha-D-ribose 1-diphosphate pH 4.8, 70°C Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.54 36200
-
2 * 36200, SDS-PAGE Methanocaldococcus jannaschii
2.4.2.54 70000
-
gel filtration Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.54 5-phospho-alpha-D-ribose 1-diphosphate + 4-aminobenzoate Methanocaldococcus jannaschii the enzyme is involved in biosynthesis of tetrahydromethanopterin, a folate analog involved in the C1 metabolism of methanogenic archaea, sulfate-reducing archaea, and methylotrophic bacteria 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.54 Methanocaldococcus jannaschii Q58822
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.54
-
Methanocaldococcus jannaschii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.54 0.248
-
pH 4.8, 70°C Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.54 5-phospho-alpha-D-ribose 1-diphosphate + 4-aminobenzoate the enzyme is involved in biosynthesis of tetrahydromethanopterin, a folate analog involved in the C1 metabolism of methanogenic archaea, sulfate-reducing archaea, and methylotrophic bacteria Methanocaldococcus jannaschii 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate
-
?
2.4.2.54 5-phospho-alpha-D-ribose 1-diphosphate + 4-aminobenzoate ordered Bi-Ter mechanism involving binding of 5-phospho-alpha-D-ribose 1-diphosphate, then 4-aminobenzoate, followed by release of the products CO2, then 4-(beta-D-ribofuranosyl)aminobenzene-5'-phosphate, and finally diphosphate Methanocaldococcus jannaschii 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.4.2.54 homodimer 2 * 36200, SDS-PAGE Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
2.4.2.54 beta-RFA-P synthase
-
Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.54 70
-
assay at Methanocaldococcus jannaschii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.54 0.23
-
5-phospho-alpha-D-ribose 1-diphosphate pH 4.8, 70°C Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.54 4.9
-
-
Methanocaldococcus jannaschii

Cofactor

EC Number Cofactor Comment Organism Structure
2.4.2.54 additional information the enzyme lacks any chromogenic cofactor, and the presence of pyridoxal phosphate and the mechanistically related pyruvoyl cofactors is excluded Methanocaldococcus jannaschii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.54 0.21
-
4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate pH 4.8, 70°C, uncompetitive inhibition under varied 5-phospho-alpha-D-ribose 1-diphosphate and saturated 4-aminobenzoate Methanocaldococcus jannaschii
2.4.2.54 0.3
-
4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate pH 4.8, 70°C, uncompetitive inhibition under varied 4-aminobenzoate and saturated 5-phospho-alpha-D-ribose 1-diphosphate Methanocaldococcus jannaschii
2.4.2.54 0.32
-
diphosphate pH 4.8, 70°C, competitive inhibition under saturated 5-phospho-alpha-D-ribose 1-diphosphate and varied 4-aminobenzoate Methanocaldococcus jannaschii
2.4.2.54 0.99
-
CO2 pH 4.8, 70°C, uncompetitive inhibition under varied 5-phospho-alpha-D-ribose 1-diphosphate and saturated 4-aminobenzoate Methanocaldococcus jannaschii