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Literature summary extracted from

  • Van Lanen, S.G.; Kinzie, S.D.; Matthieu, S.; Link, T.; Culp, J.; Iwata-Reuyl, D.
    tRNA modification by S-adenosylmethionine:tRNA ribosyltransferase-isomerase. Assay development and characterization of the recombinant enzyme (2003), J. Biol. Chem., 278, 10491-10499.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.99.17 Carbonate 100% activity in the presence of 25 mM carbonate Escherichia coli
2.4.99.17 DNA activity is highest in the presence of 0.2 mg/ml DNA Escherichia coli
2.4.99.17 EDTA 100% activity in the presence of 100 mM EDTA Escherichia coli
2.4.99.17 Sodium acetate 100% activity in the presence of 750 mM sodium acetate Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.99.17 expressed in Escherichia coli DH5apha cells Escherichia coli

General Stability

EC Number General Stability Organism
2.4.99.17 replacing Tris buffer with glycyclglycine increases activity 2fold Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.99.17 CaCl2 43% residual activity at 10 mM Escherichia coli
2.4.99.17 KCl 28% residual activity at 100 mM Escherichia coli
2.4.99.17 Mg2+ Mg2+ in millimolar concentrations inhibits enzyme activity but has little or no effect in the submillimolar range Escherichia coli
2.4.99.17 Mn2+ Mn2+ in millimolar concentrations inhibits enzyme activity but has little or no effect in the submillimolar range Escherichia coli
2.4.99.17 Na2PO4 80% residual activity at 25 mM Escherichia coli
2.4.99.17 Na2SO4 33% residual activity at 25 mM Escherichia coli
2.4.99.17 NaCl 25% residual activity at 100 mM Escherichia coli
2.4.99.17 NaSCN 60% residual activity at 0.01 mM Escherichia coli
2.4.99.17 potassium fluoride 78% residual activity at 500 mM Escherichia coli
2.4.99.17 Tris-HCl 11% residual activity at 100 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.99.17 0.0015
-
 7-aminomethyl-7-deazaguanine in tRNA(Tyr) in 100 mM glycylglycine (pH 8.7), 100 mM EDTA, 100 mM KCl, 0.5 mM dithiothreitol, at 37°C Escherichia coli
2.4.99.17 0.0377
-
 7-aminomethyl-7-deazaguanine in minihelix RNA in 100 mM glycylglycine (pH 8.7), 100 mM EDTA, 100 mM KCl, 0.5 mM dithiothreitol, at 37°C Escherichia coli
2.4.99.17 0.1014
-
 S-adenosyl-L-methionine in 100 mM glycylglycine (pH 8.7), 100 mM EDTA, 100 mM KCl, 0.5 mM dithiothreitol, at 37°C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.99.17 S-adenosyl-L-methionine + 7-aminomethyl-7-deazaguanine in tRNA(Tyr) Escherichia coli
-
 L-methionine + adenine + epoxyqueuosine in tRNA(Tyr)
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.4.99.17 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.99.17 glutathione-Sepharose column chromatography and POROS HQ column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.99.17 S-adenosyl-L-methionine + 7-aminomethyl-7-deazaguanine in minihelix RNA efficient substrate Escherichia coli L-methionine + adenine + epoxyqueuosine in minihelix RNA
-
 ir
2.4.99.17 S-adenosyl-L-methionine + 7-aminomethyl-7-deazaguanine in tRNA(Tyr)
-
 Escherichia coli L-methionine + adenine + epoxyqueuosine in tRNA(Tyr)
-
 ir

Synonyms

EC Number Synonyms Comment Organism
2.4.99.17 QueA
-
 Escherichia coli
2.4.99.17 S-adenosylmethionine:tRNA ribosyltransferase-isomerase
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.99.17 0.042
-
 S-adenosyl-L-methionine in 100 mM glycylglycine (pH 8.7), 100 mM EDTA, 100 mM KCl, 0.5 mM dithiothreitol, at 37°C Escherichia coli
2.4.99.17 0.042
-
 7-aminomethyl-7-deazaguanine in tRNA(Tyr) in 100 mM glycylglycine (pH 8.7), 100 mM EDTA, 100 mM KCl, 0.5 mM dithiothreitol, at 37°C Escherichia coli
2.4.99.17 0.245
-
 7-aminomethyl-7-deazaguanine in minihelix RNA in 100 mM glycylglycine (pH 8.7), 100 mM EDTA, 100 mM KCl, 0.5 mM dithiothreitol, at 37°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.99.17 8.7
-
 optimal activity at pH 8.7 in buffers containing various oxyanions, including acetate, carbonate, EDTA, and phosphate Escherichia coli

General Information

EC Number General Information Comment Organism
2.4.99.17 metabolism the enzyme catalyzes the penultimate step in the de novo biosynthesis of queuosine Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.99.17 6.67
-
 7-aminomethyl-7-deazaguanine in minihelix RNA in 100 mM glycylglycine (pH 8.7), 100 mM EDTA, 100 mM KCl, 0.5 mM dithiothreitol, at 37°C Escherichia coli
2.4.99.17 28.3
-
 7-aminomethyl-7-deazaguanine in tRNA(Tyr) in 100 mM glycylglycine (pH 8.7), 100 mM EDTA, 100 mM KCl, 0.5 mM dithiothreitol, at 37°C Escherichia coli