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Literature summary extracted from

  • Dunphy, J. T.; Greentree, W. K.; Linder, M. E.
    Enrichment of G-protein palmitoyltransferase activity in low density membranes (2001), J. Biol. Chem., 276, 43300-43304.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.225 additional information depletion of cellular cholesterol with the drug methyl-beta-cyclodextrin results in inhibition of palmitoyltransferase activity and a redistribution of the remaining activity to membranes of higher density, the process is reversible by cholesterol addition Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.225 membrane PAT activity is highly enriched in low density membranes dependent upon cholesterol Bos taurus 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.225 palmitoyl-CoA + [G-protein alpha subunit Galphai]-L-cysteine Bos taurus
-
 [G-protein alpha subunit Galphai]-S-palmitoyl-L-cysteine + CoA
-
 r
2.3.1.225 palmitoyl-CoA + [protein]-L-cysteine Bos taurus
-
 [protein]-S-palmitoyl-L-cysteine + CoA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.225 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.225 brain
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.225 palmitoyl-CoA + [G-protein alpha subunit Galphai]-L-cysteine
-
 Bos taurus [G-protein alpha subunit Galphai]-S-palmitoyl-L-cysteine + CoA
-
 r
2.3.1.225 palmitoyl-CoA + [protein]-L-cysteine
-
 Bos taurus [protein]-S-palmitoyl-L-cysteine + CoA
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.3.1.225 G-protein palmitoyltransferase
-
 Bos taurus
2.3.1.225 Pat
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.225 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.225 8
-
 assay at Bos taurus

General Information

EC Number General Information Comment Organism
2.3.1.225 malfunction depletion of cellular cholesterol with the drug methyl-beta-cyclodextrin results in inhibition of palmitoyltransferase activity and a redistribution of the remaining activity to membranes of higher density, the process is reversible by cholesterol addition Bos taurus
2.3.1.225 physiological function palmitoyltransferase facilitates the enrichment of fatty acylated signaling molecules in plasma membrane subdomains. Fatty acylation, involving the enzyme, is one mechanism for targeting proteins to lipid rafts, low density, sphingomyelin- and cholesterol-enriched membranes. When reconstituted into cell membranes, the population of purified recombinant Gi that is palmitoylated is highly enriched in the low density membrane fractions, whereas the bulk unmodified Gi-protein is largely excluded, the effect requires palmitoyltransferase activity and is abolished if the palmitoylated cysteine was mutated. Bos taurus