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Literature summary extracted from

  • Taura, F.; Morimoto, S.; Shoyama, Y.
    Purification and characterization of cannabidiolic-acid synthase from Cannabis sativa L.. Biochemical analysis of a novel enzyme that catalyzes the oxidocyclization of cannabigerolic acid to cannabidiolic acid (1996), J. Biol. Chem., 271, 17411-17416.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.21.3.8 Hg2+ complete inhibition at 2 mM Cannabis sativa
1.21.3.8 additional information EDTA has little influence on the enzyme activity at concentrations up to 5 mM Cannabis sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.21.3.8 0.137
-
 cannabigerolate in 100 mM sodium citrate buffer (pH 5.0), at 30°C Cannabis sativa
1.21.3.8 0.206
-
 cannabinerolate in 100 mM sodium citrate buffer (pH 5.0), at 30°C Cannabis sativa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.21.3.8 additional information the enzyme does not require metal ions Cannabis sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.21.3.8 74000
-
 1 * 74000, SDS-PAGE Cannabis sativa
1.21.3.8 75000
-
 gel filtration Cannabis sativa

Organism

EC Number Organism UniProt Comment Textmining
1.21.3.8 Cannabis sativa
-
 CBDA and Mexican strains
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.21.3.8 ammonium sulfate precipitation, DEAE-cellulose column chromatography, phenyl-Sepharose CL-4B column chromatography, and hydroxylapatite column chromatography Cannabis sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.21.3.8 leaf
-
 Cannabis sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.21.3.8 0.175
-
 enzyme from supernatant, in 100 mM sodium citrate buffer (pH 5.0), at 30°C Cannabis sativa
1.21.3.8 9
-
 after 519fold purification, in 100 mM sodium citrate buffer (pH 5.0), at 30°C Cannabis sativa

Storage Stability

EC Number Storage Stability Organism
1.21.3.8 -20°C, 40% (v/v) glycerol, ineffective storage conditions for enzyme stabilization Cannabis sativa
1.21.3.8 4°C, purified enzyme in 10 mM sodium phosphate buffer (pH 7.0), 3 mM beta-mercaptoethanol, 2 weeks, 10-20% loss of activity Cannabis sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.3.8 cannabigerolate + O2 best substrate Cannabis sativa cannabidiolate + H2O2
-
 ?
1.21.3.8 cannabinerolate + O2
-
 Cannabis sativa cannabidiolate + H2O2
-
 ?
1.21.3.8 additional information the enzyme does not require molecular oxygen for the production of cannabidiolate in the presence or absence of NADPH Cannabis sativa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.21.3.8 monomer 1 * 74000, SDS-PAGE Cannabis sativa

Synonyms

EC Number Synonyms Comment Organism
1.21.3.8 CBDA synthase
-
 Cannabis sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.21.3.8 0.03
-
 cannabinerolate in 100 mM sodium citrate buffer (pH 5.0), at 30°C Cannabis sativa
1.21.3.8 0.19
-
 cannabigerolate in 100 mM sodium citrate buffer (pH 5.0), at 30°C Cannabis sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.21.3.8 5
-
-
 Cannabis sativa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.21.3.8 4 6 half-maximal activities at pH values around 4.0 and 6.0 Cannabis sativa

Cofactor

EC Number Cofactor Comment Organism Structure
1.21.3.8 additional information the enzyme does not require any coenzyme like NAD+, NADP+, FAD, and FMN Cannabis sativa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.21.3.8 Cannabis sativa isoelectric focusing
-
 6.1