Literature summary extracted from
Umezu, N.; Umeki, N.; Mitsui, T.; Kondo, K.; Maruta, S.
Characterization of a novel rice kinesin O12 with a calponin homology domain (2011), J. Biochem., 149, 91-101.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
5.6.1.3 |
C-terminal motor domain (amino acid residues 372-717) expressed in Escherichia coli BL21(DE3) |
Oryza sativa Japonica Group |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
5.6.1.3 |
actin |
inhibition in the presence of microtubles |
Oryza sativa Japonica Group |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.6.1.3 |
Oryza sativa Japonica Group |
AK065586 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
5.6.1.3 |
immobilized metal ion affinity chromatography (Co2+) |
Oryza sativa Japonica Group |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
5.6.1.3 |
additional information |
- |
1.19 mol phosphate/kinesin mol/min in the absence of microtubles, 90.6 mol phosphate/kinesin mol/min in the presence of microtubles, Vmax/2 at 0.0037 mM microtubles, pH 7.5, 25°C |
Oryza sativa Japonica Group |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
5.6.1.3 |
ATP + H2O + a kinesin associated with a microtubule at position n |
- |
Oryza sativa Japonica Group |
ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end) |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
5.6.1.3 |
kinesin O12 |
classified as kinesin-14 family member |
Oryza sativa Japonica Group |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
5.6.1.3 |
6.5 |
- |
in the presence of microtubles |
Oryza sativa Japonica Group |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
5.6.1.3 |
5.5 |
9 |
in the presence of microtubles |
Oryza sativa Japonica Group |