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Literature summary extracted from

  • Yasumasu, S.; Kawaguchi, M.; Ouchi, S.; Sano, K.; Murata, K.; Sugiyama, H.; Akema, T.; Iuchi, I.
    Mechanism of egg envelope digestion by hatching enzymes, HCE and LCE in medaka, Oryzias latipes (2010), J. Biochem., 148, 439-448.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.66 48
-
 EVQPPDSPLSI pH 8.0, 30°C Oryzias latipes
3.4.24.67 480
-
 NPQVPQYPSKPQ pH 8.0, 30°C Oryzias latipes

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.66 Oryzias latipes
-
-
-
3.4.24.67 Oryzias latipes
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.66 embryo
-
 Oryzias latipes
-
3.4.24.67 embryo
-
 Oryzias latipes
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.66 EVQPPDSPLSI + H2O location: D387/S388 in ChgL (ZI-1,2). Specific activity: 136 nmol/30min/microgram enzyme Oryzias latipes EVQPPD + SPLSI
-
 ?
3.4.24.66 additional information cleavage sites of HCE and LCE on the egg envelope that are primarily constructed of two groups of subunit proteins, ZI-1,2 and ZI-3, are determined. LCE cleaves the middle of the zona pellucida (ZP) domain of ZI-1,2, in addition to the upstream of the trefoil domain of ZI-1,2 and the ZP domain of ZI-3. This middle site is in the intervening sequence connecting two subdomains of the ZP domain. Cleaving this site results in the solubilization of the swollen egg envelope by the disruption of the filamentous structure that is formed by the non-covalent polymerization of ZP domains Oryzias latipes ?
-
 ?
3.4.24.66 PGKNPNTPPIG + H2O location: N220/T221 in ChgH (ZI-1,2). Specific activity: 23.9 nmol/30min/microgram enzyme Oryzias latipes PGKNPN + TPPIG
-
 ?
3.4.24.66 PKLFQDGKPSN + H2O location: Q136/D137 in ChgH (ZI-1,2). Specific activity: 0.2 nmol/30min/microgram enzyme Oryzias latipes PKLFQ + DGKPSN
-
 ?
3.4.24.66 VPFEQRYPVPA + H2O location: R73/Y74 in ChgL (ZI-3). Specific activity: 76.3 nmol/30min/microgram enzyme Oryzias latipes VPFEQR + YPVPA
-
 ?
3.4.24.67 GSKTPQDPTQQ + H2O location: Q39/D40 in ChgL (ZI-3). Specific activity: 28.5 nmol/30min/microgram enzyme Oryzias latipes GSKTPQ + DPTQQ
-
 ?
3.4.24.67 additional information cleavage sites of HCE and LCE on the egg envelope that are primarily constructed of two groups of subunit proteins, ZI-1,2 and ZI-3, are determined. HCE cleaves the N-terminal region of ZI-1,2 and ZI-3, mainly the Pro-Xaa-Yaa repeat sequence of ZI-1,2 into hexapeptides, but not the site within a zona pellucida (ZP) domain that is considered to be the core structure of the egg envelope. The cleavage of the N-terminal regions results in swelling and softening of the envelope Oryzias latipes ?
-
 ?
3.4.24.67 NPQVPQYPSKPQ + H2O Pro-Xaa-Yaa repeat sequence ZI-1,2. Specific activity: 43.7 nmol/30min/microgram enzyme Oryzias latipes NPQVPQ + YPSKPQ
-
 ?
3.4.24.67 NPSYPQNPSYPQ + H2O Pro-Xaa-Yaa repeat sequence ZI-1,2. Specific activity: 81.7 nmol/30min/microgram enzyme Oryzias latipes NPSYPQ + NPSYPQ
-
 ?
3.4.24.67 PGKNPNTPPIG + H2O location: N220/T221 in ChgH (ZI-1,2). Specific activity: 11.3 nmol/30min/microgram enzyme Oryzias latipes PGKNPN + TPPIG
-
 ?
3.4.24.67 SAPVVRTSQAV + H2O synthetic substrate generated from the 25 kDa product in hatching liquid with an inefficient cleavage site Oryzias latipes NPQVPQ + YPSKPQ
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.66 LCE
-
 Oryzias latipes
3.4.24.67 HCE
-
 Oryzias latipes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.66 30
-
 assay at Oryzias latipes
3.4.24.67 30
-
 assay at Oryzias latipes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.66 8
-
 assay at Oryzias latipes
3.4.24.67 8
-
 assay at Oryzias latipes