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Literature summary extracted from

  • Hiura, H.; Matsui, T.; Matsumoto, M.; Hori, Y.; Isonishi, A.; Kato, S.; Iwamoto, T.; Mori, T.; Fujimura, Y.
    Proteolytic fragmentation and sugar chains of plasma ADAMTS13 purified by a conformation-dependent monoclonal antibody (2010), J. Biochem., 148, 403-411.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.87 Mn2+ ADAMTS13 activity is decreased in the presence of 0.9 mM Ni2+ Homo sapiens
3.4.24.87 Ni2+ ADAMTS13 activity is markedly decreased in the presence of 0.9 mM Ni2+ Homo sapiens
3.4.24.87 Zn2+ ADAMTS13 activity is markedly decreased in the presence of 0.9 mM Zn2+ Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.87 Ba2+ in the presence of 10 mM Ba2+, the activity is enhanced 1.1 to 1.4fold Homo sapiens
3.4.24.87 Ca2+ ADAMTS13 activity in the reaction buffer containing 0.9 mM Ca2+ without incubation is enhanced 2.7times compared to the value determined in the absence of Ca2+. The activity of ADAMTS13 in the presence of 10 mM Ca2+ is enhanced 3-5fold Homo sapiens
3.4.24.87 additional information the enzyme is not influenced in the presence of Mg2+ Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.87 190000
-
 x * 190000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.87 von Willebrand factor + H2O Homo sapiens ADAMTS13 specifically cleaves von Willbrand factor A2 domain between Tyr1605 and Met1606 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.87 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.24.87 glycoprotein
-
 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.87 A10-agarose column chromatography, HiTrap DEAE column chromatography, and Superdex 200 gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.87 blood plasma
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.87 additional information
-
 unpurified blood plasma ADAMTS13 has a specific activity of 0.01 units/mg, purified blood plasma ADAMTS13 has a specific activity of 300 units/mg (25057fold purification) Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.87 von Willebrand factor + H2O ADAMTS13 specifically cleaves von Willbrand factor A2 domain between Tyr1605 and Met1606 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.87 ? x * 190000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.24.87 a disintegrin-like and metalloproteinase with thrombospondin type-1 motifs 13
-
 Homo sapiens
3.4.24.87 ADAMTS13
-
 Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.87 6.1 8.8 the activity of purified pADAMTS13 is stable and relatively constant at pH ranges between pH 6.1 and 8.8 for 18 h storage at 4°C. However, ADAMTS13 activity is almost totally lost below pH 3.1 Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.24.87 Homo sapiens purified blood plasma ADAMTS13, isoelectric focusing 5.5 5.1

General Information

EC Number General Information Comment Organism
3.4.24.87 malfunction lack of blood plasma ADAMTS13 activity results in thrombotic thrombocytopenic purpura Homo sapiens