Literature summary extracted from

Ito, N.; Nureki, O.; Shirouzu, M.; Yokoyama, S.; Hanaoka, F.
Crystallization and preliminary X-ray analysis of a DNA primase from hyperthermophilic archaeon Pyrococcus horikoshii (2001), J. Biochem., 130, 727-730.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.7.7.B16	overexpression in Escherichia coli as a fusion protein with a hexa-histidine tag	Pyrococcus horikoshii

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
2.7.7.B16	hanging-drop vapor diffusion method at 20°C, with polyethylene glycol 8000 as the precipitant. The crystals belong to the P3(2)21 with unit-cell parameters a = b = 77.8, c = 129.6 A, and alpha = beta = 90°, gamma = 120°. Crystals of the selenomethionine derivative are obtained by means of a cross-seeding method using native crystals. The data for the native and selenomethionine-substituted crystals are collected to 1.8 and 2.2 A resolution	Pyrococcus horikoshii

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.7.B16	Pyrococcus horikoshii	O57934 and O57935	O57934: small subunit, O57935: large subunit	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.7.7.B16	-	Pyrococcus horikoshii

General Information

EC Number	General Information	Comment	Organism
2.7.7.B16	physiological function	at the initiation of chromosomal DNA replication, DNA primases synthesize short RNA primers, which are subsequently elongated by DNA polymerases	Pyrococcus horikoshii

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Release 2023.1 (January 2023)