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Literature summary extracted from

  • Yuan, Y.; Leeds, J.; Meredith, T.
    Pseudomonas aeruginosa directly shunts beta-oxidation degradation intermediates into de novo fatty acid biosynthesis (2012), J. Bacteriol., 194, 5185-5196.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.207 expression of orf PA3286 in Escherichia coli strain BL21(DE3) as His-tagged protein, expression of PA3286 gene in Escherichia coli strain BW25113 Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.207 additional information gene fabY is replaced with fabH (pTMT123) or deleted (pTMT124) in Pseudomonas aeruginosa strain PAO1. The fabY deletion strain grows slowly in comparison to the fabHEc exchange control without C10 supplementation, synthetic lethal analysis for fabY with KASIII domain fabH orthologues and fatty acid rescue, overview. The fabY deletion is synthetic lethal when introduced into the KASIII domain knockout strain TMT16. Synthetic lethal relationship between PA3286 and fabY indicates shared acetyl-CoA:malonyl-ACP condensing activity. PA3286 cross complements fabH and confers a fatty acid shunt in Escherichia coli Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.207 octanoyl-CoA + malonyl-[acyl-carrier protein] Pseudomonas aeruginosa PA3286 specifically shunts C8-CoA in vivo 3-oxodecanoyl-[acyl-carrier protein] + CoA + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.207 Pseudomonas aeruginosa
-
 fabY, i.e. orf PA3286
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.207 recombinant His-tagged PA3286 protein from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.207 octanoyl-CoA + malonyl-[acyl-carrier protein]
-
 Pseudomonas aeruginosa 3-oxodecanoyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.207 octanoyl-CoA + malonyl-[acyl-carrier protein] PA3286 specifically shunts C8-CoA in vivo Pseudomonas aeruginosa 3-oxodecanoyl-[acyl-carrier protein] + CoA + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.207 beta-ketoacyl synthase
-
 Pseudomonas aeruginosa
2.3.1.207 FabY
-
 Pseudomonas aeruginosa
2.3.1.207 PA3286 protein
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.207 22
-
 assay at room temperature Pseudomonas aeruginosa

General Information

EC Number General Information Comment Organism
2.3.1.207 evolution FabY belongs to the KASIII domain containing enzymes Pseudomonas aeruginosa
2.3.1.207 malfunction the fabY deletion strain grows slowly in comparison to the fabHEc exchange control without C10 supplementation Pseudomonas aeruginosa
2.3.1.207 metabolism the KASIII domain-containing enzyme FabY from open reading frame PA3286 shunts fatty acid degradation intermediates from the beta-oxidation pathway by condensing octanoyl-CoA with malonyl-ACP to make beta-keto-decanoyl-ACP, a key building block common to saturated fatty acids, unsaturated fatty acids, and lipopolysaccharides Pseudomonas aeruginosa
2.3.1.207 physiological function FabY, a beta-ketoacyl synthase KASI/II domain-containing enzyme that condenses acetyl-CoA with malonyl-acyl carrier protein (ACP) to make the fatty acid synthesis, FAS, primer beta-acetoacetyl-ACP. FabY is an enzyme required for FAS initiation in the absence of exogenous fatty acids Pseudomonas aeruginosa