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Literature summary extracted from

  • Hoffelder, M.; Raasch, K.; van Ooyen, J.; Eggeling, L.
    The E2 domain of OdhA of Corynebacterium glutamicum has succinyltransferase activity dependent on lipoyl residues of the acetyltransferase AceF (2010), J. Bacteriol., 192, 5203-5211.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.4.2 AceF acetyltransferase AceF Corynebacterium glutamicum

Protein Variants

EC Number Protein Variants Comment Organism
1.2.4.2 H352C the activity of the mutant is significantly reduced Corynebacterium glutamicum
1.2.4.2 H352C/Q356D the mutations fully prevent succinyltransferase activity Corynebacterium glutamicum
1.2.4.2 Q356D the activity of the mutant is slightly reduced Corynebacterium glutamicum
1.2.4.2 T294A the mutant shows almost no activity Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.4.2 0.13
-
 2-oxoglutarate in 50 mM TES (pH 7.7), 10 mM MgCl2, 3 mM L-cysteine, 2 mM NAD+, 0.9 mM thiamine diphosphate, 0.05 mM chlorpromazine, and 1.5 mM 2-oxoglutarate, temperature not specified in the publication Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.4.2 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine Corynebacterium glutamicum
-
 [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2
-
 ?
1.2.4.2 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine Corynebacterium glutamicum ATCC 13032
-
 [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2
-
 ?
1.2.4.2 additional information Corynebacterium glutamicum OdhA can utilize free dihydrodilipoamide to perform the E2 reaction specifically with succinyl-CoA. OdhA specifically catalyzes the E1 and E2 reaction to convert 2-oxoglutarate to succinyl-CoA but fully relies on the lipoyl residues provided by AceF involved in the reactions to convert pyruvate to acetyl-CoA ?
-
 ?
1.2.4.2 additional information Corynebacterium glutamicum ATCC 13032 OdhA can utilize free dihydrodilipoamide to perform the E2 reaction specifically with succinyl-CoA. OdhA specifically catalyzes the E1 and E2 reaction to convert 2-oxoglutarate to succinyl-CoA but fully relies on the lipoyl residues provided by AceF involved in the reactions to convert pyruvate to acetyl-CoA ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.4.2 Corynebacterium glutamicum
-
-
-
1.2.4.2 Corynebacterium glutamicum ATCC 13032
-
-
-
2.3.1.61 Corynebacterium glutamicum Q8NNJ2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.4.2 Ni-NTA column chromatography Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.4.2 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine
-
 Corynebacterium glutamicum [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2
-
 ?
1.2.4.2 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine
-
 Corynebacterium glutamicum ATCC 13032 [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2
-
 ?
1.2.4.2 additional information OdhA can utilize free dihydrodilipoamide to perform the E2 reaction specifically with succinyl-CoA. OdhA specifically catalyzes the E1 and E2 reaction to convert 2-oxoglutarate to succinyl-CoA but fully relies on the lipoyl residues provided by AceF involved in the reactions to convert pyruvate to acetyl-CoA Corynebacterium glutamicum ?
-
 ?
1.2.4.2 additional information OdhA can utilize free dihydrodilipoamide to perform the E2 reaction specifically with succinyl-CoA. OdhA specifically catalyzes the E1 and E2 reaction to convert 2-oxoglutarate to succinyl-CoA but fully relies on the lipoyl residues provided by AceF involved in the reactions to convert pyruvate to acetyl-CoA Corynebacterium glutamicum ATCC 13032 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.4.2 ODH
-
 Corynebacterium glutamicum
1.2.4.2 OdhA
-
 Corynebacterium glutamicum
1.2.4.2 oxoglutarate dehydrogenase
-
 Corynebacterium glutamicum
2.3.1.61 AceF
-
 Corynebacterium glutamicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.4.2 thiamine diphosphate dependent on Corynebacterium glutamicum

General Information

EC Number General Information Comment Organism
2.3.1.61 physiological function isolated isoform AceF has solely transacetylase activity and no transsuccinylase activity. Oxoglutarate dehydrogenase complex OdhA specifically converts 2-oxoglutarate to succinyl-coenzyme A but fully relies on the lipoyl residues provided by AceF involved in the reactions to convert pyruvate to acetyl-CoA. Presence of isoform AceF is required for both oxoglutarate dehydrogenase and pyruvate dehydrogenase activity Corynebacterium glutamicum