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Literature summary extracted from

  • Morimoto, N.; Fukuda, W.; Nakajima, N.; Masuda, T.; Terui, Y.; Kanai, T.; Oshima, T.; Imanaka, T.; Fujiwara, S.
    Dual biosynthesis pathway for longer-chain polyamines in the hyperthermophilic archaeon Thermococcus kodakarensis (2010), J. Bacteriol., 192, 4991-5001.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.104 expression in Escherichia coli Thermococcus kodakarensis
3.5.3.24 expression in Escherichia coli Thermococcus kodakarensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.104 0.012
-
 agmatine pH 9.0, 70°C Thermococcus kodakarensis
2.5.1.104 0.0807
-
 cadaverine pH 9.0, 70°C Thermococcus kodakarensis
2.5.1.104 1.053
-
 putrescine pH 9.0, 70°C Thermococcus kodakarensis
3.5.3.24 0.00642
-
 N1-aminopropylagmatine pH 7.5, 70°C Thermococcus kodakarensis
3.5.3.24 0.486
-
 agmatine pH 7.5, 70°C Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.104 S-adenosyl 3-(methylthio)propylamine + agmatine Thermococcus kodakarensis the enzyme is involved in polyamine biosynthesis 5'-S-methyl-5'-thioadenosine + N1-aminopropylagamatine
-
 ?
3.5.3.24 N1-aminopropylagmatine + H2O Thermococcus kodakarensis in Pyrococcus kodakaraensis spermidine is synthesized mainly from agmatine. The enzyme is involved in the biosynthesis of spermidine from agmatine spermidine + urea
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.104 Thermococcus kodakarensis Q5JFG9
-
-
3.5.3.24 Thermococcus kodakarensis Q5JI38
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.104
-
 Thermococcus kodakarensis
3.5.3.24
-
 Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.104 S-adenosyl 3-(methylthio)propylamine + agmatine the enzyme is involved in polyamine biosynthesis Thermococcus kodakarensis 5'-S-methyl-5'-thioadenosine + N1-aminopropylagamatine
-
 ?
2.5.1.104 S-adenosyl 3-(methylthio)propylamine + agmatine
-
 Thermococcus kodakarensis 5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
 ?
2.5.1.104 S-adenosyl 3-(methylthio)propylamine + cadaverine
-
 Thermococcus kodakarensis 5'-S-methyl-5'-thioadenosine + N-(3-aminopropyl)cadaverine
-
 ?
2.5.1.104 S-adenosyl 3-(methylthio)propylamine + putrescine
-
 Thermococcus kodakarensis 5'-S-methyl-5'-thioadenosine + spermidine
-
 ?
3.5.3.24 agmatine + H2O 43fold higher kcat/Km value for N1-aminopropylagmatine than for agmatine Thermococcus kodakarensis putrescine + urea
-
 ?
3.5.3.24 N1-aminopropylagmatine + H2O in Pyrococcus kodakaraensis spermidine is synthesized mainly from agmatine. The enzyme is involved in the biosynthesis of spermidine from agmatine Thermococcus kodakarensis spermidine + urea
-
 ?
3.5.3.24 N1-aminopropylagmatine + H2O 43fold higher kcat/Km value for N1-aminopropylagmatine than for agmatine Thermococcus kodakarensis spermidine + urea
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.104 TK0147 protein
-
 Thermococcus kodakarensis
3.5.3.24 TK0882 protein
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.104 70
-
 assay at Thermococcus kodakarensis
3.5.3.24 70
-
 assay at Thermococcus kodakarensis
3.5.3.24 90
-
-
 Thermococcus kodakarensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.3.24 90
-
 enzyme is not fully unfolded Thermococcus kodakarensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.104 0.02
-
 cadaverine pH 9.0, 70°C Thermococcus kodakarensis
2.5.1.104 0.28
-
 agmatine pH 9.0, 70°C Thermococcus kodakarensis
2.5.1.104 0.3
-
 putrescine pH 9.0, 70°C Thermococcus kodakarensis
3.5.3.24 0.001
-
 N1-aminopropylagmatine pH 7.5, 70°C Thermococcus kodakarensis
3.5.3.24 0.00186
-
 agmatine pH 7.5, 70°C Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.104 9
-
 assay at Thermococcus kodakarensis
3.5.3.24 7.5
-
 assay at Thermococcus kodakarensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.104 0.29
-
 putrescine pH 9.0, 70°C Thermococcus kodakarensis
2.5.1.104 0.29
-
 cadaverine pH 9.0, 70°C Thermococcus kodakarensis
2.5.1.104 22.5
-
 agmatine pH 9.0, 70°C Thermococcus kodakarensis
3.5.3.24 0.00387
-
 agmatine pH 7.5, 70°C Thermococcus kodakarensis
3.5.3.24 0.165
-
 N1-aminopropylagmatine pH 7.5, 70°C Thermococcus kodakarensis