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Literature summary extracted from

  • Perry, L.; Zylstra, G.
    Cloning of a gene cluster involved in the catabolism of p-nitrophenol by Arthrobacter sp. strain JS443 and characterization of the p-nitrophenol monooxygenase (2007), J. Bacteriol., 189, 7563-7572.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.166 expressed in Escherichia coli BL21(DE3)pLysS cells Arthrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.166 Arthrobacter sp. A7YVV2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.166 Ni-NTA column chromatography Arthrobacter sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.166 culture condition:4-nitrophenol-grown cell
-
 Arthrobacter sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.166 2,4,5-trichlorophenol + NADH + H+ + O2 poor substrate Arthrobacter sp. 2,5-dichlorohydroquinone + 5-chloro-2-hydroxyquinol + ? + NAD+ + H2O
-
 ?
1.14.13.166 2,4,6-trichlorophenol + NADH + H+ + O2 poor substrate Arthrobacter sp. 2,6-dichlorohydroquinone + 6-chloro-2-hydroxyquinol + ? + NAD+ + H2O
-
 ?
1.14.13.166 2,4-dinitrophenol + NADH + H+ + O2 poor substrate Arthrobacter sp. 2-nitrohydroquinone + nitrite + NAD+ + H2O
-
 ?
1.14.13.166 3-chlorophenol + NADH + H+ + O2 poor substrate Arthrobacter sp. ? + NAD+ + H2O
-
 ?
1.14.13.166 3-cresol + NADH + H+ + O2 poor substrate Arthrobacter sp. ? + NAD+ + H2O
-
 ?
1.14.13.166 3-nitrophenol + NADH + H+ + O2
-
 Arthrobacter sp. 3-nitrohydroquinone + NAD+ + H2O
-
 ?
1.14.13.166 4-chlorocatechol + NADH + H+ + O2 good substrate Arthrobacter sp. 5-chlorohydroxyquinol + ? + NAD+ + H2O
-
 ?
1.14.13.166 4-chlorophenol + NADH + H+ + O2
-
 Arthrobacter sp. 1,2,4-trihydroxybenzene + 2-hydroxy-1,4-benzoquinone + ? + NAD+ + H2O
-
 ?
1.14.13.166 4-chlororesorcinol + NADH + H+ + O2 good substrate Arthrobacter sp. 1,2,4-trihydroxybenzene + ? + NAD+ + H2O
-
 ?
1.14.13.166 4-cresol + NADH + H+ + O2 poor substrate Arthrobacter sp. 4-hydroxy-4-methyl-2,5-cyclohexadien-1-one + NAD+ + H2O
-
 ?
1.14.13.166 4-methylcatechol + NADH + H+ + O2 poor substrate Arthrobacter sp. ? + NAD+ + H2O
-
 ?
1.14.13.166 4-nitrocatechol + NADH + H+ + O2 good substrate Arthrobacter sp. 2-hydroxy-1,4-benzoquinone + nitrite + NAD+ + H2O
-
 ?
1.14.13.166 4-nitrophenol + NADH + H+ + O2 best substrate Arthrobacter sp. 2-hydroxy-1,4-benzoquinone + 1,2,4-trihydroxybenzene + nitrite + NAD+ + H2O
-
 ?
1.14.13.166 additional information hydroquinone, resorcinol, and catechol are not substrates for NpdA2 Arthrobacter sp. ?
-
 ?
1.14.13.166 phenol + NADH + H+ + O2 poor substrate Arthrobacter sp. hydroquinone + ? + NAD+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.166 NpdA2
-
 Arthrobacter sp.
1.14.13.166 p-nitrophenol monooxygenase
-
 Arthrobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.166 FAD
-
 Arthrobacter sp.
1.14.13.166 NADH
-
 Arthrobacter sp.