BRENDA - Enzyme Database

The first agmatine/cadaverine aminopropyl transferase: biochemical and structural characterization of an enzyme involved in polyamine biosynthesis in the hyperthermophilic archaeon Pyrococcus furiosus

Cacciapuoti, G.; Porcelli, M.; Moretti, M.A.; Sorrentino, F.; Concilio, L.; Zappia, V.; Liu, Z.J.; Tempel, W.; Schubot, F.; Rose, J.P.; Wang, B.C.; Brereton, P.S.; Jenney, F.E.; Adams, M.W.; J. Bacteriol. 189, 6057-6067 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.5.1.104
expression in Escherichia coli
Pyrococcus furiosus
Crystallization (Commentary)
EC Number
Crystallization
Organism
2.5.1.104
sitting drop vapor diffusion method, 1.8 A resolution
Pyrococcus furiosus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5.1.104
0.0076
-
agmatine
pH 7.4, 70C
Pyrococcus furiosus
2.5.1.104
1.88
-
propane-1,3-diamine
pH 7.4, 70C
Pyrococcus furiosus
2.5.1.104
3.77
-
cadaverine
pH 7.4, 70C
Pyrococcus furiosus
2.5.1.104
7.7
-
putrescine
pH 7.4, 70C
Pyrococcus furiosus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.5.1.104
32334
-
2 * 32334, calculated from sequence
Pyrococcus furiosus
2.5.1.104
32600
-
2 * 32600, SDS-PAGE
Pyrococcus furiosus
2.5.1.104
65000
-
gel filtration
Pyrococcus furiosus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + agmatine
Pyrococcus furiosus
agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required
5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
-
?
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + cadaverine
Pyrococcus furiosus
agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required
5'-S-methyl-5'-thioadenosine + N-(3-aminopropyl)cadaverine
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.5.1.104
Pyrococcus furiosus
Q8U4G1
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.5.1.104
-
Pyrococcus furiosus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + agmatine
agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
-
-
?
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + agmatine
4.9% of the activity compared to cadaverine
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
-
-
?
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + cadaverine
-
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + N-(3-aminopropyl)cadaverine
-
-
-
?
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + cadaverine
agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + N-(3-aminopropyl)cadaverine
-
-
-
?
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine
2.9% of the activity compared to cadaverine
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + bis(3-aminopropyl)amine
-
-
-
?
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + putrescine
8.7% of the activity compared to cadaverine
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + spermidine
-
-
-
?
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + spermidine
0.8% of the activity compared to cadaverine
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + spermine
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.5.1.104
homodimer
2 * 32334, calculated from sequence; 2 * 32600, SDS-PAGE
Pyrococcus furiosus
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.5.1.104
70
-
assay at
Pyrococcus furiosus
2.5.1.104
90
-
-
Pyrococcus furiosus
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
2.5.1.104
70
103
70C: about 50% of maximal activity, 103C: about 50% of maximal activity
Pyrococcus furiosus
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.5.1.104
108
-
transition temperature
Pyrococcus furiosus
2.5.1.104
112
-
transition temperature in presence of 5 mM cadaverine
Pyrococcus furiosus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.5.1.104
0.06
-
propane-1,3-diamine
pH 7.4, 70C
Pyrococcus furiosus
2.5.1.104
0.1
-
agmatine
pH 7.4, 70C
Pyrococcus furiosus
2.5.1.104
0.18
-
putrescine
pH 7.4, 70C
Pyrococcus furiosus
2.5.1.104
2.09
-
cadaverine
pH 7.4, 70C
Pyrococcus furiosus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.104
7.4
-
assay at
Pyrococcus furiosus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.5.1.104
expression in Escherichia coli
Pyrococcus furiosus
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.5.1.104
sitting drop vapor diffusion method, 1.8 A resolution
Pyrococcus furiosus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5.1.104
0.0076
-
agmatine
pH 7.4, 70C
Pyrococcus furiosus
2.5.1.104
1.88
-
propane-1,3-diamine
pH 7.4, 70C
Pyrococcus furiosus
2.5.1.104
3.77
-
cadaverine
pH 7.4, 70C
Pyrococcus furiosus
2.5.1.104
7.7
-
putrescine
pH 7.4, 70C
Pyrococcus furiosus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.5.1.104
32334
-
2 * 32334, calculated from sequence
Pyrococcus furiosus
2.5.1.104
32600
-
2 * 32600, SDS-PAGE
Pyrococcus furiosus
2.5.1.104
65000
-
gel filtration
Pyrococcus furiosus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + agmatine
Pyrococcus furiosus
agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required
5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
-
?
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + cadaverine
Pyrococcus furiosus
agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required
5'-S-methyl-5'-thioadenosine + N-(3-aminopropyl)cadaverine
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.5.1.104
-
Pyrococcus furiosus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + agmatine
agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
-
-
?
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + agmatine
4.9% of the activity compared to cadaverine
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
-
-
?
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + cadaverine
-
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + N-(3-aminopropyl)cadaverine
-
-
-
?
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + cadaverine
agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + N-(3-aminopropyl)cadaverine
-
-
-
?
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine
2.9% of the activity compared to cadaverine
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + bis(3-aminopropyl)amine
-
-
-
?
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + putrescine
8.7% of the activity compared to cadaverine
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + spermidine
-
-
-
?
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + spermidine
0.8% of the activity compared to cadaverine
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + spermine
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.5.1.104
homodimer
2 * 32334, calculated from sequence; 2 * 32600, SDS-PAGE
Pyrococcus furiosus
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.5.1.104
70
-
assay at
Pyrococcus furiosus
2.5.1.104
90
-
-
Pyrococcus furiosus
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
2.5.1.104
70
103
70C: about 50% of maximal activity, 103C: about 50% of maximal activity
Pyrococcus furiosus
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.5.1.104
108
-
transition temperature
Pyrococcus furiosus
2.5.1.104
112
-
transition temperature in presence of 5 mM cadaverine
Pyrococcus furiosus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.5.1.104
0.06
-
propane-1,3-diamine
pH 7.4, 70C
Pyrococcus furiosus
2.5.1.104
0.1
-
agmatine
pH 7.4, 70C
Pyrococcus furiosus
2.5.1.104
0.18
-
putrescine
pH 7.4, 70C
Pyrococcus furiosus
2.5.1.104
2.09
-
cadaverine
pH 7.4, 70C
Pyrococcus furiosus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.104
7.4
-
assay at
Pyrococcus furiosus
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.5.1.104
0.023
-
putrescine
pH 7.4, 70C
Pyrococcus furiosus
2.5.1.104
0.032
-
propane-1,3-diamine
pH 7.4, 70C
Pyrococcus furiosus
2.5.1.104
0.55
-
cadaverine
pH 7.4, 70C
Pyrococcus furiosus
2.5.1.104
13.4
-
agmatine
pH 7.4, 70C
Pyrococcus furiosus
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.5.1.104
0.023
-
putrescine
pH 7.4, 70C
Pyrococcus furiosus
2.5.1.104
0.032
-
propane-1,3-diamine
pH 7.4, 70C
Pyrococcus furiosus
2.5.1.104
0.55
-
cadaverine
pH 7.4, 70C
Pyrococcus furiosus
2.5.1.104
13.4
-
agmatine
pH 7.4, 70C
Pyrococcus furiosus