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Literature summary extracted from

  • Morii, H.; Eguchi, T.; Koga, Y.
    In vitro biosynthesis of ether-type glycolipids in the methanoarchaeon Methanothermobacter thermautotrophicus (2007), J. Bacteriol., 189, 4053-4061.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.B50 1-archaetidyl-1D-myo-inositol addition of archaetidylinositol is essential Methanothermobacter thermautotrophicus
2.4.1.B51 1-archaetidyl-1D-myo-inositol addition of archaetidylinositol is essential Methanothermobacter thermautotrophicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.B50 additional information the enzyme is concentrated in the soluble fraction Methanothermobacter thermautotrophicus
-
-
2.4.1.B51 membrane
-
 Methanothermobacter thermautotrophicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.B50 K+ stimulates, maximum activity at 0.5 M Methanothermobacter thermautotrophicus
2.4.1.B50 additional information enzyme does not require Mg2+ Methanothermobacter thermautotrophicus
2.4.1.B51 Ca2+ 5-10 mM activites 20% compared to Mg2+ Methanothermobacter thermautotrophicus
2.4.1.B51 K+ stimulates, maximum activity at 0.5 M Methanothermobacter thermautotrophicus
2.4.1.B51 Mg2+ requires 10 mM Mg2+ for maximal activity Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.B50 UDP-alpha-D-glucose + archaeol Methanothermobacter thermautotrophicus the enzyme is involved in the biosynthesis of ether-type glycolipids. The sole sugar moiety of glycolipids and phosphoglycolipids in Methanothermobacter thermautotrophicus is a gentiobiosyl group [beta-D-glucosyl-(1->6)-D-glucose]. Synthesis of the glycolipids occurs in two steps: glucose is transferred from UDP-glucose to archaeol to form monoglucosylarchaeol, followed by a second transfer to form diglucosylarchaeol UDP + 2,3-bis(O-phytanyl)-sn-glycero-1-O-beta-D-glucoside
-
 ?
2.4.1.B50 UDP-alpha-D-glucose + archaeol Methanothermobacter thermautotrophicus DSM 1053 the enzyme is involved in the biosynthesis of ether-type glycolipids. The sole sugar moiety of glycolipids and phosphoglycolipids in Methanothermobacter thermautotrophicus is a gentiobiosyl group [beta-D-glucosyl-(1->6)-D-glucose]. Synthesis of the glycolipids occurs in two steps: glucose is transferred from UDP-glucose to archaeol to form monoglucosylarchaeol, followed by a second transfer to form diglucosylarchaeol UDP + 2,3-bis(O-phytanyl)-sn-glycero-1-O-beta-D-glucoside
-
 ?
2.4.1.B51 UDP-alpha-D-glucose + 1,2-diphytanyl-sn-glycero-3-O-beta-D-glucoside Methanothermobacter thermautotrophicus the enzyme is involved in the biosynthesis of ether-type glycolipids UDP + 1,2-diphytanyl-sn-glycero-3-O-(6-O-beta-D-glucosyl)-beta-D-glucoside
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.B50 Methanothermobacter thermautotrophicus
-
-
-
2.4.1.B50 Methanothermobacter thermautotrophicus DSM 1053
-
-
-
2.4.1.B51 Methanothermobacter thermautotrophicus
-
-
-
2.4.1.B51 Methanothermobacter thermautotrophicus DSM 1053
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.B50 UDP-alpha-D-glucose + 1,2-bis-O-phytanyl-sn-glycerol 8.6% of the activity compared to archaeol Methanothermobacter thermautotrophicus UDP + ?
-
 ?
2.4.1.B50 UDP-alpha-D-glucose + 1,2-bis-O-phytanyl-sn-glycerol 8.6% of the activity compared to archaeol Methanothermobacter thermautotrophicus DSM 1053 UDP + ?
-
 ?
2.4.1.B50 UDP-alpha-D-glucose + 2,3-bis-O-geranygeranyl-sn-glycerol 29% of the activity compared to archaeol Methanothermobacter thermautotrophicus UDP + ?
-
 ?
2.4.1.B50 UDP-alpha-D-glucose + 2,3-bis-O-geranygeranyl-sn-glycerol 29% of the activity compared to archaeol Methanothermobacter thermautotrophicus DSM 1053 UDP + ?
-
 ?
2.4.1.B50 UDP-alpha-D-glucose + archaeol
-
 Methanothermobacter thermautotrophicus UDP + 2,3-bis(O-phytanyl)-sn-glycero-1-O-beta-D-glucoside
-
 ?
2.4.1.B50 UDP-alpha-D-glucose + archaeol the enzyme is involved in the biosynthesis of ether-type glycolipids. The sole sugar moiety of glycolipids and phosphoglycolipids in Methanothermobacter thermautotrophicus is a gentiobiosyl group [beta-D-glucosyl-(1->6)-D-glucose]. Synthesis of the glycolipids occurs in two steps: glucose is transferred from UDP-glucose to archaeol to form monoglucosylarchaeol, followed by a second transfer to form diglucosylarchaeol Methanothermobacter thermautotrophicus UDP + 2,3-bis(O-phytanyl)-sn-glycero-1-O-beta-D-glucoside
-
 ?
2.4.1.B50 UDP-alpha-D-glucose + archaeol
-
 Methanothermobacter thermautotrophicus DSM 1053 UDP + 2,3-bis(O-phytanyl)-sn-glycero-1-O-beta-D-glucoside
-
 ?
2.4.1.B50 UDP-alpha-D-glucose + archaeol the enzyme is involved in the biosynthesis of ether-type glycolipids. The sole sugar moiety of glycolipids and phosphoglycolipids in Methanothermobacter thermautotrophicus is a gentiobiosyl group [beta-D-glucosyl-(1->6)-D-glucose]. Synthesis of the glycolipids occurs in two steps: glucose is transferred from UDP-glucose to archaeol to form monoglucosylarchaeol, followed by a second transfer to form diglucosylarchaeol Methanothermobacter thermautotrophicus DSM 1053 UDP + 2,3-bis(O-phytanyl)-sn-glycero-1-O-beta-D-glucoside
-
 ?
2.4.1.B50 UDP-alpha-D-glucose + caldarchaeol as efficient as archaeol Methanothermobacter thermautotrophicus UDP + caldarchaeol-1-O-beta-D-glucoside
-
 ?
2.4.1.B50 UDP-alpha-D-glucose + caldarchaeol as efficient as archaeol Methanothermobacter thermautotrophicus DSM 1053 UDP + caldarchaeol-1-O-beta-D-glucoside
-
 ?
2.4.1.B50 UDP-alpha-D-glucose + caldarchaetidylinositol about 90% of the activity compared to archaeol Methanothermobacter thermautotrophicus UDP + beta-D-glucosyl caldarchaetidylinositol
-
 ?
2.4.1.B51 UDP-alpha-D-glucose + 1,2-diphytanyl-sn-glycero-3-O-beta-D-glucoside
-
 Methanothermobacter thermautotrophicus UDP + 1,2-diphytanyl-sn-glycero-3-O-(6-O-beta-D-glucosyl)-beta-D-glucoside
-
 ?
2.4.1.B51 UDP-alpha-D-glucose + 1,2-diphytanyl-sn-glycero-3-O-beta-D-glucoside the enzyme is involved in the biosynthesis of ether-type glycolipids Methanothermobacter thermautotrophicus UDP + 1,2-diphytanyl-sn-glycero-3-O-(6-O-beta-D-glucosyl)-beta-D-glucoside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.B50 archaeol:UDPglucose beta-glucosyltransferase
-
 Methanothermobacter thermautotrophicus
2.4.1.B50 MGA synthase
-
 Methanothermobacter thermautotrophicus
2.4.1.B51 DGA synthase
-
 Methanothermobacter thermautotrophicus
2.4.1.B51 MGA:UDP-glucose beta-1,6-glucosyltransferase
-
 Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.B50 60
-
 assay at Methanothermobacter thermautotrophicus
2.4.1.B51 60
-
 assay at Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.B50 8
-
-
 Methanothermobacter thermautotrophicus
2.4.1.B51 8
-
-
 Methanothermobacter thermautotrophicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.B50 6.8 8.9 pH 6.8: about 45% of maximal activity, pH 8.9: about 80% of maximal activity Methanothermobacter thermautotrophicus
2.4.1.B51 6.7 8.9 pH 6.7: about 65% of maximal activity, pH 8.9: about 50% of maximal activity Methanothermobacter thermautotrophicus