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Literature summary extracted from

  • Liu, Y.; Leal, N.A.; Sampson, E.M.; Johnson, C.L.; Havemann, G.D.; Bobik, T.A.
    PduL is an evolutionarily distinct phosphotransacylase involved in B12-dependent 1,2-propanediol degradation by Salmonella enterica serovar typhimurium LT2 (2007), J. Bacteriol., 189, 1589-1596.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.222 gene pduL, DNA and amino acid sequence determination and analysis, sequence comparison, expression as His8-tagged protein in Escherichia coli strain BE554 mostly in inclusion bodies Salmonella enterica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.222 0.61
-
 propanoyl phosphate reverse reaction, pH and temperature not specified in the publication Salmonella enterica
2.3.1.222 0.97
-
 acetyl phosphate reverse reaction, pH and temperature not specified in the publication Salmonella enterica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.222 27000
-
 x * 27000, recombinant His8-tagged PduL, SDS-PAGE Salmonella enterica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.222 propanoyl-CoA + phosphate Salmonella enterica
-
 CoA + propanoyl phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.222 Salmonella enterica
-
 serovar typhimurium LT2, diverse strains, overview, gene pduL
-
2.3.1.222 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.222 recombinant His8-tagged PduL from Escherichia coli strain BE554 soluble fraction by nickel affinity chromatographhy Salmonella enterica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.222 8.9
-
 wild-type strain, pH and temperature not specified in the publication Salmonella enterica
2.3.1.222 50
-
 recombinant His8-tagged PduL in soluble fraction of the expressing Escherichia coli strain BE554, pH and temperature not specified in the publication Salmonella enterica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.222 acetyl-CoA + phosphate
-
 Salmonella enterica CoA + acetyl phosphate
-
 r
2.3.1.222 CoA + acetyl phosphate PduL-His8 is 3.8fold more active with propionyl phosphate than with acetyl phosphate Salmonella enterica acetyl-CoA + phosphate
-
 r
2.3.1.222 CoA + propanoyl phosphate PduL-His8 is 3.8fold more active with propionyl phosphate than with acetyl phosphate Salmonella enterica propanoyl-CoA + phosphate
-
 r
2.3.1.222 propanoyl-CoA + phosphate
-
 Salmonella enterica CoA + propanoyl phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.222 ? x * 27000, recombinant His8-tagged PduL, SDS-PAGE Salmonella enterica

Synonyms

EC Number Synonyms Comment Organism
2.3.1.222 PduL
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.222 PduL
-
 Salmonella enterica
2.3.1.222 phosphotransacylase
-
 Salmonella enterica
2.3.1.222 PTAC
-
 Salmonella enterica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.222 5.4
-
 acetyl phosphate reverse reaction, pH and temperature not specified in the publication Salmonella enterica
2.3.1.222 20.7
-
 propanoyl phosphate reverse reaction, pH and temperature not specified in the publication Salmonella enterica

General Information

EC Number General Information Comment Organism
2.3.1.222 evolution PduL homologues are distributed among at least 49 bacterial species but are absent from the Archaea and Eukarya. Sequence analyses show that PduL is unrelated in amino acid sequence to known phosphotransacylase, PTAC, enzymes Salmonella enterica
2.3.1.222 malfunction pduL mutants are unable to ferment 1,2-propanediol and are also impaired for aerobic growth on this compound, overview. Ectopic expression of pduL corrects the growth defects of a pta mutant, the pta gene encodes another PTAC enzyme Salmonella enterica
2.3.1.222 metabolism PduL is required in the 1,2-propandiol degradation pathway, overview Salmonella enterica
2.3.1.222 physiological function PduL is a phosphotransacylase, PTAC, required for 1,2-propanediol degradation Salmonella enterica

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.222 5.6
-
 acetyl phosphate reverse reaction, pH and temperature not specified in the publication Salmonella enterica
2.3.1.222 33.9
-
 propanoyl phosphate reverse reaction, pH and temperature not specified in the publication Salmonella enterica