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Literature summary extracted from

  • Caccamo, M.A.; Malone, C.S.; Rasche, M.E.
    Biochemical characterization of a dihydromethanopterin reductase involved in tetrahydromethanopterin biosynthesis in Methylobacterium extorquens AM1 (2004), J. Bacteriol., 186, 2068-2073.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.47 expression in Escherichia coli as a six-histidine tag Methylorubrum extorquens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.47 17000
-
 2 * 17000, SDS-PAGE Methylorubrum extorquens
1.5.1.47 38500
-
 gel filtration Methylorubrum extorquens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.47 7,8-dihydromethanopterin + NAD(P)H + H+ Methylorubrum extorquens the enzyme is involved in tetrahydromethanopterin biosynthesis 5,6,7,8-tetrahydromethanopterin + NAD(P)+
-
 ?
1.5.1.47 7,8-dihydromethanopterin + NAD(P)H + H+ Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 the enzyme is involved in tetrahydromethanopterin biosynthesis 5,6,7,8-tetrahydromethanopterin + NAD(P)+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.47 Methylorubrum extorquens C5B2R8
-
-
1.5.1.47 Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 C5B2R8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.47
-
 Methylorubrum extorquens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.47 2.8
-
 pH 5.3, 30°C Methylorubrum extorquens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.47 7,8-dihydromethanopterin + NAD(P)H + H+ the enzyme is involved in tetrahydromethanopterin biosynthesis Methylorubrum extorquens 5,6,7,8-tetrahydromethanopterin + NAD(P)+
-
 ?
1.5.1.47 7,8-dihydromethanopterin + NAD(P)H + H+ dihydrofolate is not a substrate, no activity with reduced methyl viologen Methylorubrum extorquens 5,6,7,8-tetrahydromethanopterin + NAD(P)+
-
 ?
1.5.1.47 7,8-dihydromethanopterin + NAD(P)H + H+ the enzyme is involved in tetrahydromethanopterin biosynthesis Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 5,6,7,8-tetrahydromethanopterin + NAD(P)+
-
 ?
1.5.1.47 7,8-dihydromethanopterin + NAD(P)H + H+ dihydrofolate is not a substrate, no activity with reduced methyl viologen Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 5,6,7,8-tetrahydromethanopterin + NAD(P)+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.47 homodimer 2 * 17000, SDS-PAGE Methylorubrum extorquens

Synonyms

EC Number Synonyms Comment Organism
1.5.1.47 DmrA
-
 Methylorubrum extorquens
1.5.1.47 H2MPT reductase
-
 Methylorubrum extorquens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.47 30
-
-
 Methylorubrum extorquens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.5.1.47 20 35 20°C: about 90% of maximal activit, 35°C: about 70% of maximal activity Methylorubrum extorquens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.47 5.3
-
-
 Methylorubrum extorquens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.47 4.8 6.3 pH 4.8: about 55% of maximal activity, pH 6.3: about 70% of maximal activity Methylorubrum extorquens

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.47 NADH the specific activity with NADH is 15% of the rate observed with NADPH Methylorubrum extorquens
1.5.1.47 NADPH the specific activity with NADH is 15% of the rate observed with NADPH Methylorubrum extorquens