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Literature summary extracted from

  • Purschke, W.G.; Schmidt, C.L.; Petersen, A.; Schäfer, G.
    The terminal quinol oxidase of the hyperthermophilic archaeon Acidianus ambivalens exhibits a novel subunit structure and gene organization (1997), J. Bacteriol., 179, 1344-1353.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.1.4
-
 Acidianus ambivalens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.4 plasma membrane
-
 Acidianus ambivalens 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.1.4 Cu a heme-copper enzyme Acidianus ambivalens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.1.4 149000
-
 assuming a 1:1 stoichiometry of all encoded subunits, the Acidianus ambivalens aa3-type quinol oxidase represents a complex with a calculated molecular mass of 149000 Da. Subunits doxA and doxB later classified as EC 1.8.5.2, thiosulfate dehydrogenase (quinone) Acidianus ambivalens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.1.1.4 caldariellaquinol + O2 + n H+/in Acidianus ambivalens
-
 caldariellaquinone + H2O + n H+/out
-
 ?
7.1.1.4 caldariellaquinol + O2 + n H+/in Acidianus ambivalens DSM 3772
-
 caldariellaquinone + H2O + n H+/out
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.4 Acidianus ambivalens P94117 and P94118 P94117 (doxB, catalytic core), P94118 (doxC). Subunits doxA and doxB later classified as EC 1.8.5.2, thiosulfate dehydrogenase (quinone)
-
7.1.1.4 Acidianus ambivalens DSM 3772 P94117 and P94118 P94117 (doxB, catalytic core), P94118 (doxC). Subunits doxA and doxB later classified as EC 1.8.5.2, thiosulfate dehydrogenase (quinone)
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.4 caldariellaquinol + O2 + n H+/in
-
 Acidianus ambivalens caldariellaquinone + H2O + n H+/out
-
 ?
7.1.1.4 caldariellaquinol + O2 + n H+/in
-
 Acidianus ambivalens DSM 3772 caldariellaquinone + H2O + n H+/out
-
 ?

Subunits

EC Number Subunits Comment Organism
7.1.1.4 ? composed of at least five different subunits of 64900 Da, 38000 Da, 20400 Da, 18800 Da, and 7200 Da. Their genes are located in two different operons. While from both gene loci, a total of six genes are transcribed into mRNA, the pattern of polypeptides found in oxidase preparations comprises only four prominent protein bands on SDS-gels. The bands of 45000 Da, 40000 Da, 28000 Da, and 20000 Da can be assigned to proteins with calculated molecular masses of 38000 (DoxC), 64900 (DoxB), 18800 (DoxA), and 20400 (DoxD) Da, consistent with the frequent observation that membrane proteins display abnormal migration behavior upon denaturating SDS-PAGE. DoxB represents the catalytic core of the oxidase, exhibiting the typical consensus sequences for the heme- and copper-binding sites. Subunits doxA and doxB later classified as EC 1.8.5.2, thiosulfate dehydrogenase (quinone) Acidianus ambivalens

Synonyms

EC Number Synonyms Comment Organism
7.1.1.4 terminal quinol oxidase
-
 Acidianus ambivalens

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.4 heme a heme-copper enzyme Acidianus ambivalens