Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Roberts, D.; Zhao, S.; Doukov, T.; Ragsdale, S.
    The reductive acetyl coenzyme A pathway: Sequence and heterologous expression of active methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase from Clostridium thermoaceticum (1994), J. Bacteriol., 176, 6127-6130.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.258 expressed in Escherichia coli strain JM109 Moorella thermoacetica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.258 additional information the enzyme lacks any metals Moorella thermoacetica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.258 27000
-
 2 * 27000, SDS-PAGE Moorella thermoacetica
2.1.1.258 28550
-
 2 * 28550, recombinant enzyme, MALDI-TOF mass spectrometry Moorella thermoacetica
2.1.1.258 28640
-
 2 * 28640, native enzyme, MALDI-TOF mass spectrometry Moorella thermoacetica
2.1.1.258 28641
-
 2 * 28641, deduced from amino acid sequence Moorella thermoacetica
2.1.1.258 58900
-
 native enzyme, gel filtration Moorella thermoacetica

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.258 Moorella thermoacetica Q46389 formerly Clostridium thermoaceticum
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.258 DEAE column chromatography Moorella thermoacetica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.258 [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrofolate
-
 Moorella thermoacetica [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrofolate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.258 homodimer 2 * 27000, SDS-PAGE Moorella thermoacetica
2.1.1.258 homodimer 2 * 28550, recombinant enzyme, MALDI-TOF mass spectrometry Moorella thermoacetica
2.1.1.258 homodimer 2 * 28640, native enzyme, MALDI-TOF mass spectrometry Moorella thermoacetica
2.1.1.258 homodimer 2 * 28641, deduced from amino acid sequence Moorella thermoacetica

Synonyms

EC Number Synonyms Comment Organism
2.1.1.258 acsE
-
 Moorella thermoacetica
2.1.1.258 methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase
-
 Moorella thermoacetica
2.1.1.258 MeTr
-
 Moorella thermoacetica