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Literature summary extracted from

  • Bonting, C.F.C.; Kortstee, G.J.J.; Zehnder, A.J.B.
    Properties of polyphosphate:AMP phosphotransferase of Acinetobacter strain 210A (1991), J. Bacteriol., 173, 6484-6488.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.33 ATP 6 mM, 30% inhibition Acinetobacter sp.
2.7.4.33 diphosphate
-
 Acinetobacter johnsonii
2.7.4.33 diphosphate
-
 Acinetobacter sp.
2.7.4.33 EDTA
-
 Acinetobacter johnsonii
2.7.4.33 Tetraphosphate
-
 Acinetobacter johnsonii
2.7.4.33 Tetraphosphate mixed type inhibition with polyphosphate as substrate Acinetobacter sp.
2.7.4.33 Triphosphate
-
 Acinetobacter johnsonii
2.7.4.33 Triphosphate mixed type inhibition with polyphosphate as substrate Acinetobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.33 0.0008
-
 (Phosphate)n pH and temperature not specified in the publication, polyphosphate with an average chain length of 35 phosphate groups Acinetobacter sp.
2.7.4.33 0.0008
-
 [phosphate]n polyphosphate with an average chain length of 35 phosphate groups, pH 8.5, 30°C Acinetobacter johnsonii
2.7.4.33 0.26
-
 AMP pH 8.5, 30°C Acinetobacter johnsonii
2.7.4.33 0.26
-
 AMP pH and temperature not specified in the publication Acinetobacter sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.33 Mg2+ required Acinetobacter johnsonii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.33 55000
-
 x * 55000, SDS-PAGE Acinetobacter sp.
2.7.4.33 55000
-
 x * 55000, SDS-PAGE Acinetobacter johnsonii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.33 AMP + [phosphate]n Acinetobacter johnsonii
-
 ADP + [phosphate]n-1
-
 ?
2.7.4.33 AMP + [phosphate]n Acinetobacter johnsonii 210A
-
 ADP + [phosphate]n-1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.33 Acinetobacter johnsonii
-
-
-
2.7.4.33 Acinetobacter johnsonii 210A
-
-
-
2.7.4.33 Acinetobacter sp.
-
-
-
2.7.4.33 Acinetobacter sp. 210A
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.33
-
 Acinetobacter sp.
2.7.4.33 native enzyme 1511fold from strain 210A by streptomycin sulfate precipitation, two steps of anion exchange chromatography, hydrophobic interaction chromatography, and gel filtration Acinetobacter johnsonii

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.4.33 ADP + (phosphate)n = AMP + (phosphate)n+1 the mechanism of polyphosphate degradation by polyphosphate:AMP phosphotransferase is processive. Once bound, the polyphosphate chain does not dissociate from the enzyme until it has been utilized completely Acinetobacter johnsonii
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.33 101.2
-
 purified native enzyme, pH 8.5, 30°C Acinetobacter johnsonii

Storage Stability

EC Number Storage Stability Organism
2.7.4.33 -20°C, 1 month, about 50% loss of activity which is not due to freezing and thawing Acinetobacter sp.
2.7.4.33 -20°C, purified enzyme, 1 month, 50% activity remaining, activity loss is not due to freezing and thawing Acinetobacter johnsonii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.33 AMP + (phosphate)n highest activity are found with polyphosphate molecules of 18 to 44 phosphate residues. The polyphosphate chain is degraded completely to ADP. The mechanism of the degradation is processive. No activity is obtained with ortho-, di-, tri-, and tetraphosphate Acinetobacter sp. ADP + (phosphate)n-1
-
 ?
2.7.4.33 AMP + (phosphate)n highest activity are found with polyphosphate molecules of 18 to 44 phosphate residues. The polyphosphate chain is degraded completely to ADP. The mechanism of the degradation is processive. No activity is obtained with ortho-, di-, tri-, and tetraphosphate Acinetobacter sp. 210A ADP + (phosphate)n-1
-
 ?
2.7.4.33 AMP + [phosphate]n
-
 Acinetobacter johnsonii ADP + [phosphate]n-1
-
 ?
2.7.4.33 AMP + [phosphate]n polyphosphate with an average chain length of 35 phosphate groups is the best substrate Acinetobacter johnsonii ADP + [phosphate]n-1
-
 ?
2.7.4.33 AMP + [phosphate]n
-
 Acinetobacter johnsonii 210A ADP + [phosphate]n-1
-
 ?
2.7.4.33 AMP + [phosphate]n polyphosphate with an average chain length of 35 phosphate groups is the best substrate Acinetobacter johnsonii 210A ADP + [phosphate]n-1
-
 ?
2.7.4.33 additional information no activity with ortho-, di-, tri-, and tetraphosphates, highest activities with polyphosphate of a degree of polymerization between 18 and 44 phosphate groups, overview Acinetobacter johnsonii ?
-
 ?
2.7.4.33 additional information no activity with ortho-, di-, tri-, and tetraphosphates, highest activities with polyphosphate of a degree of polymerization between 18 and 44 phosphate groups, overview Acinetobacter johnsonii 210A ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.33 ? x * 55000, SDS-PAGE Acinetobacter sp.
2.7.4.33 ? x * 55000, SDS-PAGE Acinetobacter johnsonii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.33 30
-
 assay at Acinetobacter johnsonii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.33 30
-
 2 h, stable Acinetobacter sp.
2.7.4.33 30
-
 purified enzyme, 2 h, completely stable Acinetobacter johnsonii
2.7.4.33 45
-
 15 min, 50% loss of activity Acinetobacter sp.
2.7.4.33 45
-
 purified enzyme, 15 min, loss of 50% activity Acinetobacter johnsonii
2.7.4.33 50
-
 15 min, complete loss of activity Acinetobacter sp.
2.7.4.33 50
-
 purified enzyme, inactivation Acinetobacter johnsonii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.33 6.5 8.5
-
 Acinetobacter sp.
2.7.4.33 6.5 8.5
-
 Acinetobacter johnsonii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.33 5.5 10 no activity is observed below pH 5.5 and above pH 10 Acinetobacter sp.
2.7.4.33 5.5 10 no activity below pH 5.5 and above pH 10.0 Acinetobacter johnsonii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.33 additional information
-
 additional information inhibition kinetics, overview Acinetobacter johnsonii