EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.329 | expression in Escherichia coli | Streptomyces fradiae |
2.6.1.100 | gene neoB, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli | Streptomyces fradiae |
2.6.1.101 | gene neoB, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli | Streptomyces fradiae |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.329 | Zn2+ | assay in presence of | Streptomyces fradiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.100 | L-glutamine + 2-deoxy-scyllo-inosose | Streptomyces fradiae | - |
2-oxoglutaramate + 2-deoxy-scyllo-inosamine | - |
? | |
2.6.1.100 | L-glutamine + 2-deoxy-scyllo-inosose | Streptomyces fradiae NBRC 12773 | - |
2-oxoglutaramate + 2-deoxy-scyllo-inosamine | - |
? | |
2.6.1.101 | L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose | Streptomyces fradiae | - |
2-oxoglutaramate + 2-deoxystreptamine | - |
? | |
2.6.1.101 | L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose | Streptomyces fradiae NBRC 12773 | - |
2-oxoglutaramate + 2-deoxystreptamine | - |
? | |
2.6.1.101 | additional information | Streptomyces fradiae | NeoB also shows L-glutamine:2-deoxy-scyllo-inosose aminotransferase activity resulting in formation of 2-deoxy-scyllo-inosamine, EC 2.6.1.100 | ? | - |
? | |
2.6.1.101 | additional information | Streptomyces fradiae NBRC 12773 | NeoB also shows L-glutamine:2-deoxy-scyllo-inosose aminotransferase activity resulting in formation of 2-deoxy-scyllo-inosamine, EC 2.6.1.100 | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.329 | Streptomyces fradiae | Q53U21 | - |
- |
1.1.1.329 | Streptomyces fradiae NBRC 12773 | Q53U21 | - |
- |
2.6.1.100 | Streptomyces fradiae | Q53U20 | gene neoB, encoded in the neomycin biosynthetic gene cluster | - |
2.6.1.100 | Streptomyces fradiae NBRC 12773 | Q53U20 | gene neoB, encoded in the neomycin biosynthetic gene cluster | - |
2.6.1.101 | Streptomyces fradiae | Q53U20 | gene neoB, encoded in the neomycin biosynthetic gene cluster | - |
2.6.1.101 | Streptomyces fradiae NBRC 12773 | Q53U20 | gene neoB, encoded in the neomycin biosynthetic gene cluster | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.329 | 2-deoxy-scyllo-inosamine + NAD+ | - |
Streptomyces fradiae | 3-amino-2,3-dideoxy-scyllo-inosose + NADH + H+ | - |
? | |
1.1.1.329 | 2-deoxy-scyllo-inosamine + NAD+ | - |
Streptomyces fradiae NBRC 12773 | 3-amino-2,3-dideoxy-scyllo-inosose + NADH + H+ | - |
? | |
1.1.1.329 | 2-deoxy-scyllo-inosamine + NADP+ | - |
Streptomyces fradiae | 3-amino-2,3-dideoxy-scyllo-inosose + NADPH + H+ | - |
? | |
1.1.1.329 | 2-deoxy-scyllo-inosamine + NADP+ | - |
Streptomyces fradiae NBRC 12773 | 3-amino-2,3-dideoxy-scyllo-inosose + NADPH + H+ | - |
? | |
2.6.1.100 | L-glutamine + 2-deoxy-scyllo-inosose | - |
Streptomyces fradiae | 2-oxoglutaramate + 2-deoxy-scyllo-inosamine | - |
? | |
2.6.1.100 | L-glutamine + 2-deoxy-scyllo-inosose | - |
Streptomyces fradiae NBRC 12773 | 2-oxoglutaramate + 2-deoxy-scyllo-inosamine | - |
? | |
2.6.1.100 | additional information | NeoB also shows L-glutamine:3-amino-2,3-dideoxy-scyllo-inosose aminotransferase activity resulting in formation of 2-deoxy-streptamine, EC 2.6.1.101 | Streptomyces fradiae | ? | - |
? | |
2.6.1.100 | additional information | NeoB also shows L-glutamine:3-amino-2,3-dideoxy-scyllo-inosose aminotransferase activity resulting in formation of 2-deoxy-streptamine, EC 2.6.1.101 | Streptomyces fradiae NBRC 12773 | ? | - |
? | |
2.6.1.101 | L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose | - |
Streptomyces fradiae | 2-oxoglutaramate + 2-deoxystreptamine | - |
? | |
2.6.1.101 | L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose | - |
Streptomyces fradiae NBRC 12773 | 2-oxoglutaramate + 2-deoxystreptamine | - |
? | |
2.6.1.101 | additional information | NeoB also shows L-glutamine:2-deoxy-scyllo-inosose aminotransferase activity resulting in formation of 2-deoxy-scyllo-inosamine, EC 2.6.1.100 | Streptomyces fradiae | ? | - |
? | |
2.6.1.101 | additional information | NeoB also shows L-glutamine:2-deoxy-scyllo-inosose aminotransferase activity resulting in formation of 2-deoxy-scyllo-inosamine, EC 2.6.1.100 | Streptomyces fradiae NBRC 12773 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.329 | neoA | - |
Streptomyces fradiae |
2.6.1.100 | Neo6 | - |
Streptomyces fradiae |
2.6.1.100 | neoB | - |
Streptomyces fradiae |
2.6.1.101 | Gln:DOI aminotransferase | - |
Streptomyces fradiae |
2.6.1.101 | L-glutamine:DOI aminotransferase | - |
Streptomyces fradiae |
2.6.1.101 | Neo6 | - |
Streptomyces fradiae |
2.6.1.101 | neoB | - |
Streptomyces fradiae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.101 | 28 | - |
assay at | Streptomyces fradiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.101 | 8 | - |
assay at | Streptomyces fradiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.329 | NAD+ | enzyme accepts both NAD+ and NADP+ | Streptomyces fradiae | |
1.1.1.329 | NADP+ | enzyme accepts both NAD+ and NADP+ | Streptomyces fradiae | |
2.6.1.100 | pyridoxal 5'-phosphate | - |
Streptomyces fradiae | |
2.6.1.101 | pyridoxal 5'-phosphate | - |
Streptomyces fradiae |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.6.1.100 | metabolism | the enzyme is involved in the biosynthesis of 2-deoxystreptamine (DOS)-containing aminoglycoside neomycin. NeoB also shows L-glutamine:3-amino-2,3-dideoxy-scyllo-inosose aminotransferase activity resulting in formation of 2-deoxy-streptamine, EC 2.6.1.101 | Streptomyces fradiae |
2.6.1.100 | additional information | the equivalent of gene neoB, from neo gene cluster, in the btr gene cluster is gene btrS | Streptomyces fradiae |
2.6.1.101 | metabolism | the enzyme is involved in the biosynthesis of the 2-deoxystreptamine (DOS)-containing aminoglycoside neomycin. 3-Amino-2,3-dideoxy-scyllo-inosose produced by the NeoA reaction is continuously used as substrate for the natural forward transamination catalyzed by NeoB in the 2-deoxystreptamine biosynthesis. NeoB also shows L-glutamine:2-deoxy-scyllo-inosose aminotransferase activity resulting in formation of 2-deoxy-scyllo-inosamine, EC 2.6.1.100 | Streptomyces fradiae |
2.6.1.101 | additional information | the equivalent of gene neoB, from neo gene cluster, in the btr gene cluster is gene btrS | Streptomyces fradiae |