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Literature summary extracted from

  • Kudo, F.; Yamauchi, N.; Suzuki, R.; Kakinuma, K.
    Kinetic isotope effect and reaction mechanism of 2-deoxy-scyllo-inosose synthase derived from butirosin-producing Bacillus circulans (1997), J. Antibiot., 50, 424-428.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.124 Co2+ required Niallia circulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.124 D-glucose 6-phosphate Niallia circulans intramolecular carbocyclization, mechanism, overview 2-deoxy-L-scyllo-inosose + phosphate
-
 ?
4.2.3.124 D-glucose 6-phosphate Niallia circulans SANK72073 intramolecular carbocyclization, mechanism, overview 2-deoxy-L-scyllo-inosose + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.124 Niallia circulans Q9S5E2 gene btrC, a butirosin producing strain
-
4.2.3.124 Niallia circulans SANK72073 Q9S5E2 gene btrC, a butirosin producing strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.124 native enzyme by ammonium sulfate fractionation, dialysis and two different steps of anion exchange chromatography Niallia circulans

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.3.124 D-glucose 6-phosphate = 2-deoxy-L-scyllo-inosose + phosphate reaction mechanism, overview Niallia circulans
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.124 D-glucose 6-phosphate intramolecular carbocyclization, mechanism, overview Niallia circulans 2-deoxy-L-scyllo-inosose + phosphate
-
 ?
4.2.3.124 D-glucose 6-phosphate intramolecular carbocyclization, hydride abstraction and returning appeared to take place within the same glucose molecule Niallia circulans 2-deoxy-L-scyllo-inosose + phosphate product identification by GC-MS analysis ?
4.2.3.124 D-glucose 6-phosphate intramolecular carbocyclization, mechanism, overview Niallia circulans SANK72073 2-deoxy-L-scyllo-inosose + phosphate
-
 ?
4.2.3.124 D-glucose 6-phosphate intramolecular carbocyclization, hydride abstraction and returning appeared to take place within the same glucose molecule Niallia circulans SANK72073 2-deoxy-L-scyllo-inosose + phosphate product identification by GC-MS analysis ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.124 DOI synthase
-
 Niallia circulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.124 37
-
 assay at Niallia circulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.124 7.5
-
 assay at Niallia circulans

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.3.124 NAD+ essential for activity Niallia circulans

General Information

EC Number General Information Comment Organism
4.2.3.124 physiological function the enzyme is crucial in the carbocyclization reaction as part of the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics. The DOI synthase catalyzes the intramolecular carbocyclization of D-glucose 6-phosphate into the first non-aminogenous cyclitol 2-deoxy-L-scyllo-inosose, DOI Niallia circulans