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Literature summary extracted from

  • Cooke, H.A.; Peck, S.C.; Evans, B.S.; van der Donk, W.A.
    Mechanistic investigation of methylphosphonate synthase, a non-heme iron-dependent oxygenase (2012), J. Am. Chem. Soc., 134, 15660-15663.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.73 0.0045
-
 2-hydroxyethylphosphonate temperature not specified in the publication, pH not specified in the publication Nitrosopumilus maritimus
1.13.11.73 0.0065
-
 (S)-2-hydroxyethylphosphonate temperature not specified in the publication, pH not specified in the publication Nitrosopumilus maritimus
1.13.11.73 0.0071
-
 (R)-2-hydroxyethylphosphonate temperature not specified in the publication, pH not specified in the publication Nitrosopumilus maritimus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.73 Iron non-heme iron-dependent enzyme Nitrosopumilus maritimus

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.73 Nitrosopumilus maritimus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.73 (R)-2-hydroxyethylphosphonate + O2
-
 Nitrosopumilus maritimus methylphosphonate + HCO3-
-
 ?
1.13.11.73 (S)-2-hydroxyethylphosphonate + O2
-
 Nitrosopumilus maritimus methylphosphonate + HCO3-
-
 ?
1.13.11.73 2-hydroxyethylphosphonate + O2 stereospecific hydrogen transfer of the pro-R hydrogen at C2 of the substrate to the methyl group of methylphosphonate. Neither hydrogen transfer is rate limiting under saturating substrate conditions Nitrosopumilus maritimus methylphosphonate + HCO3-
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.73 mpnS
-
 Nitrosopumilus maritimus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.73 0.17
-
 (S)-2-hydroxyethylphosphonate temperature not specified in the publication, pH not specified in the publication Nitrosopumilus maritimus
1.13.11.73 0.17
-
 (R)-2-hydroxyethylphosphonate temperature not specified in the publication, pH not specified in the publication Nitrosopumilus maritimus
1.13.11.73 0.18
-
 2-hydroxyethylphosphonate temperature not specified in the publication, pH not specified in the publication Nitrosopumilus maritimus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.73 24
-
 (R)-2-hydroxyethylphosphonate temperature not specified in the publication, pH not specified in the publication Nitrosopumilus maritimus
1.13.11.73 26
-
 (S)-2-hydroxyethylphosphonate temperature not specified in the publication, pH not specified in the publication Nitrosopumilus maritimus
1.13.11.73 40
-
 2-hydroxyethylphosphonate temperature not specified in the publication, pH not specified in the publication Nitrosopumilus maritimus