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Literature summary extracted from

  • Kudo, F.; Kawabe, K.; Kuriki, H.; Eguchi, T.; Kakinuma, K.
    A new family of glucose-1-phosphate/glucosamine-1-phosphate nucleotidylyltransferase in the biosynthetic pathways for antibiotics (2005), J. Am. Chem. Soc., 127, 1711-1718.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.24 expressed in Escherichia coli Niallia circulans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.24 Ca2+ 5.5 mM, 2.1% activity Niallia circulans
2.7.7.24 Co2+ 5.5 mM, 124% activity Niallia circulans
2.7.7.24 Cu2+ 5.5 mM, 0.6% activity Niallia circulans
2.7.7.24 Fe2+ 5.5 mM, 0.3% activity Niallia circulans
2.7.7.24 Mg2+ 5.5 mM, 100% activity Niallia circulans
2.7.7.24 Mn2+ 5.5 mM, 98.5% activity Niallia circulans
2.7.7.24 Ni2+ 5.5 mM, 97.1% activity Niallia circulans
2.7.7.24 Zn2+ 5.5 mM, 17.9% activity Niallia circulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.24 31810
-
 mass spectroscopy Niallia circulans

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.24 Niallia circulans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.24 D-glucosamine 1-phosphate + TTP
-
 Niallia circulans TTP-D-glucosamine + diphosphate
-
 ?
2.7.7.24 D-glucosamine 1-phosphate + UTP
-
 Niallia circulans UDP-D-glucosamine + diphosphate
-
 ?
2.7.7.24 D-glucose 1-phosphate + UTP
-
 Niallia circulans UDP-D-glucose + diphosphate
-
 ?
2.7.7.24 additional information BtrD is inactive with ATP, GTP, or CTP Niallia circulans ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.24 37
-
 assay at Niallia circulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.24 7.5
-
 assay at Niallia circulans

General Information

EC Number General Information Comment Organism
2.7.7.24 malfunction btrD mutant shows no production of any antibiotics under the standard fermentation conditions, while the complementation of this disruption by transformation recovers the antibiotic production Niallia circulans
2.7.7.24 physiological function btrD gene is involved in the butirosin biosynthesis Niallia circulans