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Literature summary extracted from

  • Taura, F.; Morimoto, S.; Shoyama, Y.; Mechoulam, R.
    First direct evidence for the mechanism of DELTA1-tetrahydrocannabinolic acid biosynthesis (1995), J. Am. Chem. Soc., 117, 9766-9767.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.21.3.7 Fe3+ 91% residual activity at 2 mM Fe3+ Cannabis sativa
1.21.3.7 Hg2+ strong inhibitor, 4% residual activity at 2 mM Hg2+ Cannabis sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.21.3.7 0.134
-
 cannabigerolate at pH 6.0, temperature not specified in the publication Cannabis sativa
1.21.3.7 0.254
-
 cannabinerolate at pH 6.0, temperature not specified in the publication Cannabis sativa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.21.3.7 additional information the enzyme is not influenced by Ca2+, Mg2+ and Mn2+ Cannabis sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.21.3.7 74000
-
 gel filtration Cannabis sativa
1.21.3.7 75000
-
 1 * 75000, SDS-PAGE Cannabis sativa

Organism

EC Number Organism UniProt Comment Textmining
1.21.3.7 Cannabis sativa
-
 Mexican strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.21.3.7 diethylaminoethyl-cellulose column chromatography, phenyl Sepharose CL-4B column chromatography, and hydroxylapatite column chromatography Cannabis sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.21.3.7 leaf bud
-
 Cannabis sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.3.7 cannabigerolate + O2 best substrate Cannabis sativa DELTA9-tetrahydrocannabinolate + H2O2
-
 ?
1.21.3.7 cannabinerolate + O2
-
 Cannabis sativa DELTA9-tetrahydrocannabinolate + H2O2
-
 ?
1.21.3.7 additional information cannabigerol and cannabinero are no substrates Cannabis sativa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.21.3.7 monomer 1 * 75000, SDS-PAGE Cannabis sativa

Synonyms

EC Number Synonyms Comment Organism
1.21.3.7 DELA1-THCA synthase
-
 Cannabis sativa
1.21.3.7 DELTA1-tetrahydrocannabinolic acid synthase
-
 Cannabis sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.21.3.7 5.5 6
-
 Cannabis sativa

Cofactor

EC Number Cofactor Comment Organism Structure
1.21.3.7 additional information NAD+, NADP+, FAD, and FMN do not stimulate the enzyme activity Cannabis sativa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.21.3.7 Cannabis sativa isoelectric focusing
-
 6.4