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Literature summary extracted from

  • Whitman, C.; Hajipour, G.; Watson, R.; Johnson Jr., W.; Bembenek, M.; Stolowich, N.
    Stereospecific ketonization of 2-hydroxymuconate by 4-oxalocrotonate tautomerase and 5-(carboxymethyl)-2-hydroxymuconate isomerase (1992), J. Am. Chem. Soc., 114, 10104-10110.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.2.6 14000
-
 x * 14000, 5-(carboxymethyl)-2-hydroxymuconate isomerase, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
5.3.2.6 Escherichia coli
-
-
-
5.3.2.6 Escherichia coli C
-
-
-
5.3.2.6 Pseudomonas putida
-
-
-
5.3.2.6 Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.2.6 native enzyme from Escherichia coli strain C by ammonium sulfate fractionation, gel filtration, anion exchange chromatography, ultrafiltration, ammoniums ulfate fractionation, and hydrophobic interaction chromatography, followed by gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.2.6 2-hydroxymuconate stereospecific ketonization Pseudomonas putida 2-oxo-3-(E)-hexenedioate
-
 ?
5.3.2.6 2-hydroxymuconate stereospecific ketonization, a reaction of EC 5.3.2.6, is also catalyzed by 5-(carboxymethyl)-2-hydroxymuconate isomerase, EC 5.3.3.10 Escherichia coli 2-oxo-3-(E)-hexenedioate
-
 ?
5.3.2.6 2-hydroxymuconate stereospecific ketonization Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 2-oxo-3-(E)-hexenedioate
-
 ?
5.3.2.6 2-hydroxymuconate stereospecific ketonization, a reaction of EC 5.3.2.6, is also catalyzed by 5-(carboxymethyl)-2-hydroxymuconate isomerase, EC 5.3.3.10 Escherichia coli C 2-oxo-3-(E)-hexenedioate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.2.6 ? x * 14000, 5-(carboxymethyl)-2-hydroxymuconate isomerase, SDS-PAGE Escherichia coli
5.3.2.6 ? x * 3600, 4-oxalocrotonate tautomerase, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
5.3.2.6 4-OT
-
 Pseudomonas putida
5.3.2.6 4-oxalocrotonate tautomerase
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.2.6 30
-
 assay at Escherichia coli
5.3.2.6 30
-
 assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.2.6 7.3
-
 assay at Escherichia coli
5.3.2.6 7.3
-
 assay at Pseudomonas putida

General Information

EC Number General Information Comment Organism
5.3.2.6 evolution mechanism and the evolution of 4-oxalocrotonate tautomerase, and 5-(carboxymethyl)-2-hydroxymuconate isomerase, EC 5.3.3.10, and their respective pathways, overview Pseudomonas putida
5.3.2.6 evolution mechanism and the evolution of 4-oxalocrotonate tautomerase, EC 5.3.2.6, and 5-(carboxymethyl)-2-hydroxymuconate isomerase and their respective pathways, overview Escherichia coli
5.3.2.6 metabolism in the catechol meta-fission pathway elaborated by Pseudomonas putida mt-2 ketonization of 2-hydroxymuconate by 4-oxalocrotonate tautomerase generates the alpha,beta-unsaturated ketone 2-oxo-3-(E)-hexenedioate, which undergoes decarboxylation and further processing to intermediates in the Krebs cycle Pseudomonas putida
5.3.2.6 metabolism in the homoprotocatechuate pathway elaborated by Escherichia coli C ketonization of 5-(carboxymethyl)-2-hydroxymuconate by 5-(carboxymethyl)-2-hydroxymuconate isomerase generates the alpha,beta-unsaturated ketone, which undergoes decarboxylation and further processing to intermediates in the Krebs cycle Escherichia coli