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Literature summary extracted from
- Expression, identification and purification of Dictyostelium acetoacetyl-CoA thiolase expressed in Escherichia coli (2011), Int. J. Biol. Sci., 7, 9-17.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.3.1.9 | expression in Escherichia coli | Dictyostelium discoideum |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.9 | 43000 | - |
x * 43445, calculated, x * 43000, SDS-PAGE | Dictyostelium discoideum |
| 2.3.1.9 | 43445 | - |
x * 43445, calculated, x * 43000, SDS-PAGE | Dictyostelium discoideum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.3.1.9 | Dictyostelium discoideum | Q86AD9 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.3.1.9 | recombinant protein | Dictyostelium discoideum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.9 | 4.7 | - |
30°C, pH 8.3 | Dictyostelium discoideum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.3.1.9 | acetoacetyl-CoA + CoA | - |
Dictyostelium discoideum | 2 acetyl-CoA | - |
? |  |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.3.1.9 | ? | x * 43445, calculated, x * 43000, SDS-PAGE | Dictyostelium discoideum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.9 | 40 | - |
stable up to | Dictyostelium discoideum |
| 2.3.1.9 | 60 | - |
10 min, 50% residual activity | Dictyostelium discoideum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.9 | 8 | 8.5 | - |
Dictyostelium discoideum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.9 | 7 | 9 | significant decrease in activiy below pH 7.0 or above pH 9.0 | Dictyostelium discoideum |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 2.3.1.9 | Dictyostelium discoideum | calculated | - |
8.4 |
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