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Literature summary extracted from

  • Mehere, P.; Han, Q.; Christensen, B.M.; Li, J.
    Identification and characterization of two arylalkylamine N-acetyltransferases in the yellow fever mosquito, Aedes aegypti (2011), Insect Biochem. Mol. Biol., 41, 707-714.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.87 aaNAT1-4, aaNAT5b, aaNAT7, aaNAT9, and aaNAT11, homology sequence analysis, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, expression profiling of aaNAT1 aaNAT2 based on real time quantitative reverse transcriptase PCR, individual recombinant expression Aedes aegypti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.87 additional information
-
 additional information Michaelis-Menten kinetic analysis Aedes aegypti
2.3.1.87 0.13
-
 octopamine pH 7.3, 22°C, recombinant aaNAT2 Aedes aegypti
2.3.1.87 0.14
-
 octopamine pH 7.3, 22°C, recombinant aaNAT1 Aedes aegypti
2.3.1.87 0.16
-
 tryptamine pH 7.3, 22°C, recombinant aaNAT1 Aedes aegypti
2.3.1.87 0.16
-
 norepinephrine pH 7.3, 22°C, recombinant aaNAT1 Aedes aegypti
2.3.1.87 0.17
-
 tyramine pH 7.3, 22°C, recombinant aaNAT1 Aedes aegypti
2.3.1.87 0.17
-
 tryptamine pH 7.3, 22°C, recombinant aaNAT2 Aedes aegypti
2.3.1.87 0.17
-
 norepinephrine pH 7.3, 22°C, recombinant aaNAT2 Aedes aegypti
2.3.1.87 0.18
-
 methoxytryptamine pH 7.3, 22°C, recombinant aaNAT1 Aedes aegypti
2.3.1.87 0.19
-
 dopamine pH 7.3, 22°C, recombinant aaNAT1 Aedes aegypti
2.3.1.87 0.23
-
 serotonin pH 7.3, 22°C, recombinant aaNAT1 Aedes aegypti
2.3.1.87 0.23
-
 tyramine pH 7.3, 22°C, recombinant aaNAT2 Aedes aegypti
2.3.1.87 0.23
-
 methoxytryptamine pH 7.3, 22°C, recombinant aaNAT2 Aedes aegypti
2.3.1.87 0.27
-
 dopamine pH 7.3, 22°C, recombinant aaNAT2 Aedes aegypti
2.3.1.87 0.42
-
 serotonin pH 7.3, 22°C, recombinant aaNAT2 Aedes aegypti

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.87 acetyl-CoA + dopamine Aedes aegypti
-
 CoA + N-acetyldopamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.87 Aedes aegypti
-
 aaNAT1-4, aaNAT5b, aaNAT7, aaNAT9, and aaNAT11
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.87 recombinant aaNAT1-4, aaNAT5b, aaNAT7, aaNAT9, and aaNAT11 by chinin affinity and ion exchange chromatography, and gel filtration Aedes aegypti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.87 acetyl-CoA + dopamine
-
 Aedes aegypti CoA + N-acetyldopamine
-
 ?
2.3.1.87 acetyl-CoA + methoxytryptamine
-
 Aedes aegypti CoA + N-acetylmethoxytryptamine
-
 ?
2.3.1.87 acetyl-CoA + norepinephrine
-
 Aedes aegypti CoA + N-acetylnorepinephrine
-
 ?
2.3.1.87 acetyl-CoA + octopamine
-
 Aedes aegypti CoA + N-acetyloctopamine
-
 ?
2.3.1.87 acetyl-CoA + serotonin i.e. 5-hydroxytryptamine Aedes aegypti CoA + N-acetylserotonin
-
 ?
2.3.1.87 acetyl-CoA + tryptamine
-
 Aedes aegypti CoA + N-acetyltryptamine
-
 ?
2.3.1.87 acetyl-CoA + tyramine
-
 Aedes aegypti CoA + N-acetyltyramine
-
 ?
2.3.1.87 additional information substrate specificity, aaNAT1 and aaNAT2 show a broad substrate specificity and their affinity and catalytic efficiency to each of the seven arylalkylamines tested. The affinity between aaNAT1 and aaNAT2 to most of the arylalkylamines is similar except that aaNAT2 has less affinity than aaNAT1 to norepinephrine. Also aaNAT1 is more efficient in catalyzing all the tested substrates than that of aaNAT2 Aedes aegypti ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.87 ? x * 20000-35000, aaNAT1-4, aaNAT5b, aaNAT7, aaNAT9, and aaNAT11, SDS-PAGE Aedes aegypti

Synonyms

EC Number Synonyms Comment Organism
2.3.1.87 NAT
-
 Aedes aegypti
2.3.1.87 PaaNAT
-
 Aedes aegypti

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.87 22
-
 assay at room temperature Aedes aegypti

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.87 2.56
-
 octopamine pH 7.3, 22°C, recombinant aaNAT2 Aedes aegypti
2.3.1.87 3.05
-
 tryptamine pH 7.3, 22°C, recombinant aaNAT2 Aedes aegypti
2.3.1.87 3.16
-
 tyramine pH 7.3, 22°C, recombinant aaNAT2 Aedes aegypti
2.3.1.87 3.17
-
 norepinephrine pH 7.3, 22°C, recombinant aaNAT2 Aedes aegypti
2.3.1.87 3.47
-
 serotonin pH 7.3, 22°C, recombinant aaNAT2 Aedes aegypti
2.3.1.87 3.65
-
 dopamine pH 7.3, 22°C, recombinant aaNAT2 Aedes aegypti
2.3.1.87 3.74
-
 methoxytryptamine pH 7.3, 22°C, recombinant aaNAT2 Aedes aegypti
2.3.1.87 6.775
-
 tyramine pH 7.3, 22°C, recombinant aaNAT1 Aedes aegypti
2.3.1.87 7.26
-
 octopamine pH 7.3, 22°C, recombinant aaNAT1 Aedes aegypti
2.3.1.87 7.3
-
 dopamine pH 7.3, 22°C, recombinant aaNAT1 Aedes aegypti
2.3.1.87 7.345
-
 tryptamine pH 7.3, 22°C, recombinant aaNAT1 Aedes aegypti
2.3.1.87 7.545
-
 serotonin pH 7.3, 22°C, recombinant aaNAT1 Aedes aegypti
2.3.1.87 7.69
-
 methoxytryptamine pH 7.3, 22°C, recombinant aaNAT1 Aedes aegypti
2.3.1.87 7.76
-
 norepinephrine pH 7.3, 22°C, recombinant aaNAT1 Aedes aegypti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.87 7.3
-
 assay at Aedes aegypti

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.87 acetyl-CoA
-
 Aedes aegypti