EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.101 | gene legI or Cj1327, DNA and ammino acid sequence determination and analysis, expression as N-terminally His6-tagged enzyme | Campylobacter jejuni |
2.7.7.82 | His-tagged protein expressed in Escherichia coli | Campylobacter jejuni |
3.2.1.184 | expressed as a His-tagged fusion protein | Campylobacter jejuni |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.101 | 2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O | Campylobacter jejuni | - |
N,N'-diacetyllegionaminate + phosphate | - |
? | |
2.5.1.101 | 2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O | Campylobacter jejuni 11168 | - |
N,N'-diacetyllegionaminate + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.101 | Campylobacter jejuni | - |
gene legI or Cj1327 | - |
2.5.1.101 | Campylobacter jejuni 11168 | - |
gene legI or Cj1327 | - |
2.7.7.82 | Campylobacter jejuni | - |
- |
- |
2.7.7.82 | Campylobacter jejuni 11168 | - |
- |
- |
3.2.1.184 | Campylobacter jejuni | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.101 | recombinant N-terminally His6-tagged enzyme by nickel affinity chromatography | Campylobacter jejuni |
2.7.7.82 | immobilized metal ion affinity chromatography (Ni2+) | Campylobacter jejuni |
3.2.1.184 | using Ni-NTA chromatography | Campylobacter jejuni |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.101 | 2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O | - |
Campylobacter jejuni | N,N'-diacetyllegionaminate + phosphate | - |
? | |
2.5.1.101 | 2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O | - |
Campylobacter jejuni 11168 | N,N'-diacetyllegionaminate + phosphate | - |
? | |
2.7.7.82 | CTP + N,N'-diacetyllegionaminic acid | - |
Campylobacter jejuni | CMP-N,N'-diacetyllegionaminic acid + diphosphate | - |
? | |
2.7.7.82 | CTP + N,N'-diacetyllegionaminic acid | - |
Campylobacter jejuni 11168 | CMP-N,N'-diacetyllegionaminic acid + diphosphate | - |
? | |
3.2.1.184 | GDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucose + H2O | enzyme performs C2-epimerization resulting in NDP removal. Enzyme does not react with UDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucose, but when the quantity of LegG is 10fold increased within this reaction, UDP removal is observed | Campylobacter jejuni | ? | - |
? | |
3.2.1.184 | GDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucose + H2O | enzyme performs C2-epimerization resulting in NDP removal. Enzyme does not react with UDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucose, but when the quantity of LegG is 10fold increased within this reaction, UDP removal is observed | Campylobacter jejuni 11168 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.7.82 | ? | x * about 30000, His-tagged protein, SDS-PAGE | Campylobacter jejuni |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.101 | legI | - |
Campylobacter jejuni |
2.7.7.82 | CMP-legionaminic acid synthetase | - |
Campylobacter jejuni |
2.7.7.82 | legF | - |
Campylobacter jejuni |
3.2.1.184 | Cj1328 | - |
Campylobacter jejuni |
3.2.1.184 | LeG | - |
Campylobacter jejuni |
3.2.1.184 | neuC | - |
Campylobacter jejuni |
3.2.1.184 | UDP-N-acetylglucosamine 2-epimerase | - |
Campylobacter jejuni |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.101 | 25 | - |
assay at | Campylobacter jejuni |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.101 | 7.3 | - |
assay at | Campylobacter jejuni |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.5.1.101 | metabolism | the enzyme is part of the biosynthesis of legionaminic acid and its CMP-activated form, metabolite determination and identification by NMR spectroscoopy for pathway construction and analysis, overview | Campylobacter jejuni |