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Literature summary extracted from

  • Schoenhofen, I.; Vinogradov, E.; Whitfield, D.; Brisson, J.; Logan, S.
    The CMP-legionaminic acid pathway in Campylobacter: Biosynthesis involving novel GDP-linked precursors (2009), Glycobiology, 19, 715-725.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.101 gene legI or Cj1327, DNA and ammino acid sequence determination and analysis, expression as N-terminally His6-tagged enzyme Campylobacter jejuni
2.7.7.82 His-tagged protein expressed in Escherichia coli Campylobacter jejuni
3.2.1.184 expressed as a His-tagged fusion protein Campylobacter jejuni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.101 2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O Campylobacter jejuni
-
 N,N'-diacetyllegionaminate + phosphate
-
 ?
2.5.1.101 2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O Campylobacter jejuni 11168
-
 N,N'-diacetyllegionaminate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.101 Campylobacter jejuni
-
 gene legI or Cj1327
-
2.5.1.101 Campylobacter jejuni 11168
-
 gene legI or Cj1327
-
2.7.7.82 Campylobacter jejuni
-
-
-
2.7.7.82 Campylobacter jejuni 11168
-
-
-
3.2.1.184 Campylobacter jejuni
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.101 recombinant N-terminally His6-tagged enzyme by nickel affinity chromatography Campylobacter jejuni
2.7.7.82 immobilized metal ion affinity chromatography (Ni2+) Campylobacter jejuni
3.2.1.184 using Ni-NTA chromatography Campylobacter jejuni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.101 2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O
-
 Campylobacter jejuni N,N'-diacetyllegionaminate + phosphate
-
 ?
2.5.1.101 2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O
-
 Campylobacter jejuni 11168 N,N'-diacetyllegionaminate + phosphate
-
 ?
2.7.7.82 CTP + N,N'-diacetyllegionaminic acid
-
 Campylobacter jejuni CMP-N,N'-diacetyllegionaminic acid + diphosphate
-
 ?
2.7.7.82 CTP + N,N'-diacetyllegionaminic acid
-
 Campylobacter jejuni 11168 CMP-N,N'-diacetyllegionaminic acid + diphosphate
-
 ?
3.2.1.184 GDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucose + H2O enzyme performs C2-epimerization resulting in NDP removal. Enzyme does not react with UDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucose, but when the quantity of LegG is 10fold increased within this reaction, UDP removal is observed Campylobacter jejuni ?
-
 ?
3.2.1.184 GDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucose + H2O enzyme performs C2-epimerization resulting in NDP removal. Enzyme does not react with UDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucose, but when the quantity of LegG is 10fold increased within this reaction, UDP removal is observed Campylobacter jejuni 11168 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.82 ? x * about 30000, His-tagged protein, SDS-PAGE Campylobacter jejuni

Synonyms

EC Number Synonyms Comment Organism
2.5.1.101 legI
-
 Campylobacter jejuni
2.7.7.82 CMP-legionaminic acid synthetase
-
 Campylobacter jejuni
2.7.7.82 legF
-
 Campylobacter jejuni
3.2.1.184 Cj1328
-
 Campylobacter jejuni
3.2.1.184 LeG
-
 Campylobacter jejuni
3.2.1.184 neuC
-
 Campylobacter jejuni
3.2.1.184 UDP-N-acetylglucosamine 2-epimerase
-
 Campylobacter jejuni

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.101 25
-
 assay at Campylobacter jejuni

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.101 7.3
-
 assay at Campylobacter jejuni

General Information

EC Number General Information Comment Organism
2.5.1.101 metabolism the enzyme is part of the biosynthesis of legionaminic acid and its CMP-activated form, metabolite determination and identification by NMR spectroscoopy for pathway construction and analysis, overview Campylobacter jejuni