Literature summary extracted from

Ito, N.; Nureki, O.; Shirouzu, M.; Yokoyama, S.; Hanaoka, F.
Crystal structure of the Pyrococcus horikoshii DNA primase-UTP complex: implications for the mechanism of primer synthesis (2003), Genes Cells, 8, 913-923.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.7.7.B16	hexa-histidine tagged enzyme is expressed in Escherichia coli	Pyrococcus horikoshii

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
2.7.7.B16	cocrystalization with uridine 5'-triphosphate allowing confirmation of the location of the active site. Construction of a model between the DNA primase and a primer/template DNA based on the complex structure for the primer synthesis	Pyrococcus horikoshii

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.7.B16	Pyrococcus horikoshii	O57934 and O57935	O57934: small subunit, O57935: large subunit	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.7.7.B16	-	Pyrococcus horikoshii

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Release 2023.1 (January 2023)