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Literature summary extracted from

  • Fukuda, W.; Morimoto, N.; Imanaka, T.; Fujiwara, S.
    Agmatine is essential for the cell growth of Thermococcus kodakaraensis (2008), FEMS Microbiol. Lett., 287, 113-120.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.19 expressed in Escherichia coli Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.19 12600
-
6 * 12600 + 6 * 4500, (alphabeta)6 complex, denaturant gel electrophoresis Thermococcus kodakarensis
4.1.1.19 99400
-
gel filtration Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.19 L-arginine Thermococcus kodakarensis first step of polyamine biosynthesis agmatine + CO2
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.19 Thermococcus kodakarensis Q5JFI4
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.1.1.19 proteolytic modification synthesized as an inactive proenzyme Thermococcus kodakarensis
4.1.1.19 pyruvoyl group formation the enzyme is synthesized as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue (Ser44) via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme Thermococcus kodakarensis

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.19 recombinant enzyme Thermococcus kodakarensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.19 0.28
-
pH 6.0, 70-90°C Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.19 L-arginine first step of polyamine biosynthesis Thermococcus kodakarensis agmatine + CO2
-
?
4.1.1.19 L-arginine no activity with ornithine and lysine Thermococcus kodakarensis agmatine + CO2
-
?

Subunits

EC Number Subunits Comment Organism
4.1.1.19 hexamer 6 * 12600 + 6 * 4500, (alphabeta)6 complex, denaturant gel electrophoresis Thermococcus kodakarensis

Synonyms

EC Number Synonyms Comment Organism
4.1.1.19 pyruvoyl-dependent arginine decarboxylase
-
Thermococcus kodakarensis
4.1.1.19 Tk-PdaD
-
Thermococcus kodakarensis
4.1.1.19 TK0149
-
Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.19 70 90 assay at Thermococcus kodakarensis
4.1.1.19 100
-
-
Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.19 6
-
assay at Thermococcus kodakarensis

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.19 additional information pyruvoyl-dependent decarboxylase, synthesised as zymogen. The pyruvoyl cofactor results from the self-modification of an internal serine (Ser44) residue of the proenzyme, the pyruvoyl group functions through the formation of a Schiff base with the substrate to promote decarboxylation Thermococcus kodakarensis

General Information

EC Number General Information Comment Organism
4.1.1.19 physiological function a gene disruptant lacking arginine decarboxylase is constructed, showing that it grows only in the medium in the presence of agmatine but not in the absence of agmatine. The results indicates that agmatine is essential for the cell growth of Thermococcus kodakaraensis Thermococcus kodakarensis